Western Blot (WB)
(Combined immunoprecipitation and western blot using EGFR antibody. Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15'' at 100 ng/ml and without (-) the addition of EGF. The combination of immunoprecipitation and western blotting was performed using the anti-EGFR antibody for immunoprecipitation (10 ul) followed by western blot detection using an anti-phosphotyrosine antibody (Panel A). This was repeated in reverse order using a 1:2000 dilution of anti-EGFR for western blot (Panel B). Visualization occurred using an ECL system. Film exposure was approximately 1'. Other detection systems will yield similar results.)
Rabbit EGFR Polyclonal Antibody | anti-EGFR antibody
EGFR antibody
Reactivity
To be determined by end-user
Applications
ELISA, Immunoprecipitation, Western Blot
Synonyms
EGFR; Polyclonal Antibody; EGFR antibody; Polyclonal EGFR; Anti-EGFR; egfr; Epidermal Growth FactorEgf receptor; egf-r; v-erb-b homolog; epidermal growth factor receptor; panitumumab; erbb1; anti-EGFR antibody
Host
Rabbit
Reactivity
To be determined by end-user
Clonality
Polyclonal
Form/Format
Supplied as a liquid with 0.01% NaN3.
Concentration
85.0 mg/ml (varies by lot)
Sequence Length
10
Applicable Applications for anti-EGFR antibody
ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
ELISA: 1:10,000-1:50,000
IP: 2.5 ug/ml
WB: 1:1,000-1:10,000
IP: 2.5 ug/ml
WB: 1:1,000-1:10,000
Biohazard Information
This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.
Immunogen
EGFR antibody was raised in rabbit using a synthetic peptide corresponding to a region near the carboxy terminus which is identical in human, mouse and rat EGFR, as the immunogen.
Preparation and Storage
Store at 4 degree C. Dilute only prior to immediate use.
Western Blot (WB)
(Combined immunoprecipitation and western blot using EGFR antibody. Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15'' at 100 ng/ml and without (-) the addition of EGF. The combination of immunoprecipitation and western blotting was performed using the anti-EGFR antibody for immunoprecipitation (10 ul) followed by western blot detection using an anti-phosphotyrosine antibody (Panel A). This was repeated in reverse order using a 1:2000 dilution of anti-EGFR for western blot (Panel B). Visualization occurred using an ECL system. Film exposure was approximately 1'. Other detection systems will yield similar results.)
Western Blot (WB)
(Combined immunoprecipitation and western blot using EGFR antibody. Lysates were prepared from GN4 rat liver epithelial cells both with (+) EGF treatment for 15'' at 100 ng/ml and without (-) the addition of EGF. The combination of immunoprecipitation and western blotting was performed using the anti-EGFR antibody for immunoprecipitation (10 ul) followed by western blot detection using an anti-phosphotyrosine antibody (Panel A). This was repeated in reverse order using a 1:2000 dilution of anti-EGFR for western blot (Panel B). Visualization occurred using an ECL system. Film exposure was approximately 1'. Other detection systems will yield similar results.)
Related Product Information for anti-EGFR antibody
Rabbit polyclonal EGFR antibody
Product Categories/Family for anti-EGFR antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
1,020 Da
NCBI Official Full Name
epidermal growth factor receptor, partial
UniProt Protein Name
Epidermal growth factor receptor
Protein Family
UniProt Gene Name
c-erbB proto-oncogene
UniProt Entry Name
Q9P2Z9_HUMAN
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Product Notes
The EGFR c-erbb proto-oncogene (Catalog #AAA534141) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EGFR antibody reacts with To be determined by end-user and may cross-react with other species as described in the data sheet. AAA Biotech's EGFR can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunoprecipitation (IP), Western Blot (WB). ELISA: 1:10,000-1:50,000 IP: 2.5 ug/ml WB: 1:1,000-1:10,000. Researchers should empirically determine the suitability of the EGFR c-erbb proto-oncogene for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EGFR, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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