Rabbit EFNA5 Polyclonal Antibody | anti-EFNA5 antibody

EFNA5 Polyclonal Antibody

Cat. Num. AAA2522317

• Gene Names: EFNA5; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Immunohistochemistry
• Purity: Antigen affinity purification
• Synonyms: EFNA5; Polyclonal Antibody; EFNA5 Polyclonal Antibody; AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7; anti-EFNA5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen affinity purification
• Concentration: 2.5mg/mL (varies by lot)
• Sequence Length: 228

• Applicable Applications for anti-EFNA5 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic peptide of human EFNA5
• Buffer: PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Preparation and Storage: Store at -20 degree C (regular) and -80 degree C (long term). Avoid freeze / thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using EFNA5 Polyclonal Antibody at dilution 1:40)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Human colon cancer tissue using EFNA5 Polyclonal Antibody at dilution 1:40)

Related Product Information for anti-EFNA5 antibody

Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 1706678
• NCBI GeneID: 1946
• Molecular Weight: 26,297 Da
• NCBI Official Full Name: Ephrin-A5
• NCBI Official Synonym Full Names: ephrin-A5
• NCBI Official Symbol: EFNA5
• NCBI Official Synonym Symbols: AF1; EFL5; RAGS; EPLG7; GLC1M; LERK7
• NCBI Protein Information: ephrin-A5; AL-1; LERK-7; eph-related receptor tyrosine kinase ligand 7
• UniProt Protein Name: Ephrin-A5
• Protein Family: Ephrin
• UniProt Gene Name: EFNA5
• UniProt Synonym Gene Names: EPLG7; LERK7; LERK-7
• UniProt Entry Name: EFNA5_HUMAN

NCBI Description

Ephrin-A5, a member of the ephrin gene family, prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin ligands and receptors have been named by the Eph Nomenclature Committee (1997). Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

Uniprot Description

EFNA5: Cell surface GPI-bound ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Induces compartmentalized signaling within a caveolae-like membrane microdomain when bound to the extracellular domain of its cognate receptor. This signaling event requires the activity of the Fyn tyrosine kinase. Activates the EPHA3 receptor to regulate cell-cell adhesion and cytoskeletal organization. With the receptor EPHA2 may regulate lens fiber cells shape and interactions and be important for lens transparency maintenance. May function actively to stimulate axon fasciculation. The interaction of EFNA5 with EPHA5 also mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion. Cognate/functional ligand for EPHA7, their interaction regulates brain development modulating cell-cell adhesion and repulsion. Belongs to the ephrin family.

Protein type: Cell development/differentiation; Ligand, receptor tyrosine kinase; Motility/polarity/chemotaxis; Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 5q21

Cellular Component: anchored to external side of plasma membrane; extracellular region; plasma membrane; caveola

Molecular Function: ephrin receptor binding; chemorepellent activity

Biological Process: nervous system development; axon guidance; positive regulation of peptidyl-tyrosine phosphorylation; regulation of cell-cell adhesion; apoptosis; regulation of actin cytoskeleton organization and biogenesis; regulation of focal adhesion formation; ephrin receptor signaling pathway; negative chemotaxis; retinal ganglion cell axon guidance