Western Blot (WB)
(Mouse Eef2k Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Eef2k antibody detected the Eef2k protein (arrow).)
Rabbit anti-Mouse Eef2k Polyclonal Antibody | anti-Eef2k antibody
Eef2k, NT (Eef2k, Eukaryotic elongation factor 2 kinase, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase) (APC)
Gene Names
Eef2k; C86191; eEF-2K
Reactivity
Mouse
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Eef2k; Polyclonal Antibody; NT (Eef2k; Eukaryotic elongation factor 2 kinase; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase) (APC); Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase; anti-Eef2k antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Sequence Length
724
Applicable Applications for anti-Eef2k antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Mouse Eef2k antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 177-205 amino acids from the N-terminal region of Mouse Eef2k.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Mouse Eef2k Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Eef2k antibody detected the Eef2k protein (arrow).)
Western Blot (WB)
(Mouse Eef2k Antibody (N-term) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Eef2k antibody detected the Eef2k protein (arrow).)
Related Product Information for anti-Eef2k antibody
Phosphorylates eukaryotic elongation factor-2. Binds calmodulin (By similarity).
Product Categories/Family for anti-Eef2k antibody
References
Khan, A.A., et al. Am. J. Pathol. 177(1):481-492(2010). Shen, Q., et al. Parkinsonism Relat. Disord. 15(7):525-531(2009). Py, B.F., et al. Autophagy 5(3):393-396(2009). Im, H.I., et al. PLoS ONE 4 (10), E7424 (2009): Crawley, S.W., et al. Biochim. Biophys. Acta 1784(6):908-915(2008).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
eukaryotic elongation factor 2 kinase
NCBI Official Synonym Full Names
eukaryotic elongation factor-2 kinase
NCBI Official Symbol
Eef2k
NCBI Official Synonym Symbols
C86191; eEF-2K
NCBI Protein Information
eukaryotic elongation factor 2 kinase
UniProt Protein Name
Eukaryotic elongation factor 2 kinase
Protein Family
UniProt Gene Name
Eef2k
UniProt Synonym Gene Names
eEF-2 kinase; eEF-2K
UniProt Entry Name
EF2K_MOUSE
Research Articles on Eef2k
Similar Products
Product Notes
The Eef2k eef2k (Catalog #AAA6294457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Eef2k, NT (Eef2k, Eukaryotic elongation factor 2 kinase, Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase) (APC) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Eef2k can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Eef2k eef2k for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Eef2k, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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