Rabbit eEF2 Polyclonal Antibody | anti-eEF2 antibody

eEF2 (Phospho-Thr57) Antibody

Cat. Num. AAA9429231

• Gene Names: EEF2; EF2; EF-2; EEF-2; SCA26
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Synonyms: eEF2; Polyclonal Antibody; eEF2 (Phospho-Thr57) Antibody; EEF 2; Eef2; EF-2; EF2; EF2_HUMAN; Elongation factor 2; Eukaryotic translation elongation factor 2; Polypeptidyl tRNA translocase; SCA26;; anti-eEF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-eEF2 (Phospho-Thr57) Antibody detects endogenous levels of eEF2 only when phosphorylated at Thr57
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Form/Format: In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0mg/mL (varies by lot)
• Sequence Length: 858

• Applicable Applications for anti-eEF2 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human eEF2 around the phosphorylation site of Thr57
• Immunogen Type: Peptide
• Preparation and Storage: Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Phospho-eEF2 (Thr57) in lysates of NIH-3T3 Serum 10% 30', using Phospho-eEF2 (Thr57) Antibody(13079).)

Product Categories/Family for anti-eEF2 antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 4503483
• NCBI GeneID: 1938
• NCBI Accession #: NP_001952.1
• NCBI GenBank Nucleotide #: NM_001961.3
• UniProt Accession #: P13639; Q58J86; B2RMP5; D6W618
• Molecular Weight: 95
• NCBI Official Full Name: elongation factor 2
• NCBI Official Synonym Full Names: eukaryotic translation elongation factor 2
• NCBI Official Symbol: EEF2
• NCBI Official Synonym Symbols: EF2; EF-2; EEF-2; SCA26
• NCBI Protein Information: elongation factor 2
• UniProt Protein Name: Elongation factor 2
• Protein Family: Elongation factor
• UniProt Gene Name: EEF2
• UniProt Synonym Gene Names: EF2; EF-2

NCBI Description

This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.