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SDS-Page (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: HepG2, 293T and Hela cell, Human fetal liver tissue lysates, Primary antibody:ECH1 Antibody at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds)

Rabbit anti-Human, Rat ECH1 Polyclonal Antibody | anti-ECH1 antibody

ECH1 Antibody

Gene Names
ECH1; HPXEL
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Antigen affinity purification
Synonyms
ECH1; Polyclonal Antibody; ECH1 Antibody; HPXEL; anti-ECH1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Purity/Purification
Antigen affinity purification
Form/Format
pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Concentration
0.9mg/mL (varies by lot)
Applicable Applications for anti-ECH1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:300
Immunogen
Fusion protein of human ECH1
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C for 1 year.

SDS-Page

(Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: HepG2, 293T and Hela cell, Human fetal liver tissue lysates, Primary antibody:ECH1 Antibody at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds)

SDS-Page (Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-4: HepG2, 293T and Hela cell, Human fetal liver tissue lysates, Primary antibody:ECH1 Antibody at dilution 1/300, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds)

Immunohistochemistry (IHC)

(The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (ECH1 Antibody) at dilution 1/50.)

Immunohistochemistry (IHC) (The image is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (ECH1 Antibody) at dilution 1/50.)
Related Product Information for anti-ECH1 antibody
This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans, 5-cis-dienoyl-CoA to 2-trans, 4-trans-dienoyl-CoA, indicating that it is a delta3, 5-delta2, 4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.
Product Categories/Family for anti-ECH1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
35,816 Da
NCBI Official Full Name
Homo sapiens enoyl Coenzyme A hydratase 1, peroxisomal, mRNA
NCBI Official Synonym Full Names
enoyl-CoA hydratase 1
NCBI Official Symbol
ECH1
NCBI Official Synonym Symbols
HPXEL
NCBI Protein Information
delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
UniProt Protein Name
Doublesex- and mab-3-related transcription factor A2
UniProt Gene Name
DMRTA2
UniProt Synonym Gene Names
DMRT5
UniProt Entry Name
DMTA2_HUMAN

NCBI Description

This gene encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators. [provided by RefSeq, Jul 2008]

Research Articles on ECH1

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Product Notes

The ECH1 dmrta2 (Catalog #AAA9434693) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ECH1 Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ECH1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:300. Researchers should empirically determine the suitability of the ECH1 dmrta2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ECH1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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