Western Blot (WB)
(EBPL Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the EBPL antibody detected the EBPL protein (arrow).)
Rabbit anti-Human EBPL Polyclonal Antibody | anti-EBPL antibody
EBPL, CT (EBPL, EBRP, ERP, Emopamil-binding protein-like, Emopamil-binding-related protein) (FITC)
Gene Names
EBPL; EBRP
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
EBPL; Polyclonal Antibody; CT (EBPL; EBRP; ERP; Emopamil-binding protein-like; Emopamil-binding-related protein) (FITC); Emopamil-binding-related protein; anti-EBPL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-EBPL antibody
Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
FC/FACS: 1:10-50
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
IHC: 1:50-100
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
EBPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-214 amino acids from the C-terminal region of human EBPL.
Conjugate
FITC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(EBPL Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the EBPL antibody detected the EBPL protein (arrow).)
Western Blot (WB)
(EBPL Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (15ug/lane).This demonstrates the EBPL antibody detected the EBPL protein (arrow).)
Immunohistochemistry (IHC)
(EBPL Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EBPL Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(EBPL Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EBPL Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(EBPL Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(EBPL Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-EBPL antibody
EBPL does not possess sterol isomerase activity and does not bind sigma ligands.
Product Categories/Family for anti-EBPL antibody
References
Ma, J., et al. Atherosclerosis 191(1):63-72(2007). Moebius, F.F., et al. Biochem. J. 374 (PT 1), 229-237 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,204 Da
NCBI Official Full Name
emopamil-binding protein-like isoform 1
NCBI Official Synonym Full Names
emopamil binding protein-like
NCBI Official Symbol
EBPL
NCBI Official Synonym Symbols
EBRP
NCBI Protein Information
emopamil-binding protein-like; emopamil-binding-related protein; emopamil binding related protein, delta8-delta7 sterol isomerase related protein
UniProt Protein Name
Emopamil-binding protein-like
Protein Family
UniProt Gene Name
EBPL
UniProt Synonym Gene Names
EBRP; ERP
UniProt Entry Name
EBPL_HUMAN
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Product Notes
The EBPL ebpl (Catalog #AAA6294206) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EBPL, CT (EBPL, EBRP, ERP, Emopamil-binding protein-like, Emopamil-binding-related protein) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's EBPL can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB). FC/FACS: 1:10-50 FLISA: 1:1,000 IHC: 1:50-100 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the EBPL ebpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EBPL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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