Rabbit anti-Human EBP1 Polyclonal Antibody | anti-EBP1 antibody

EBP1, ID (Proliferation-associated Protein 2G4, Cell Cycle Protein p38-2G4 Homolog, hG4-1, ErbB3-binding Protein 1, PA2G4, EBP1) (MaxLight 750)

Cat. Num. AAA6473473

• Gene Names: PA2G4; EBP1; HG4-1; p38-2G4
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: EBP1; Polyclonal Antibody; ID (Proliferation-associated Protein 2G4; Cell Cycle Protein p38-2G4 Homolog; hG4-1; ErbB3-binding Protein 1; PA2G4; EBP1) (MaxLight 750); anti-EBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human EBP1.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-EBP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human EBP1 (KLH).
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(EBP1 Antibody (Center) western blot analysis in A2058,Jurkat,MCF-7 cell line lysates (35ug/lane).This demonstrates the EBP1 antibody detected the EBP1 protein (arrow).)

Immunohistochemistry (IHC)

(EBP1 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the EBP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(EBP1 Antibody (Center) flow cytometric analysis of MCF-7 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-EBP1 antibody

Antibodies; Binding Proteins

References

1. Zhang, Y., Mol. Cancer Ther. 7(10), 3176-3186 (2008). 2. Zhang, Y., Cancer Lett. 265(2), 298-306 (2008). 3. Okada, M., J. Biol. Chem. 282(50), 36744-36754 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 124494254
• NCBI GeneID: 5036
• NCBI Accession #: NP_006182
• NCBI GenBank Nucleotide #: NM_006191.2
• UniProt Accession #: Q9UQ80; O43846; Q9UM59
• Molecular Weight: 38,059 Da
• NCBI Official Full Name: proliferation-associated protein 2G4
• NCBI Official Synonym Full Names: proliferation-associated 2G4
• NCBI Official Symbol: PA2G4
• NCBI Official Synonym Symbols: EBP1; HG4-1; p38-2G4
• NCBI Protein Information: proliferation-associated protein 2G4
• UniProt Protein Name: Proliferation-associated protein 2G4
• Protein Family: ERBB-3 BINDING protein
• UniProt Gene Name: PA2G4
• UniProt Synonym Gene Names: EBP1; hG4-1
• UniProt Entry Name: PA2G4_HUMAN

NCBI Description

This gene encodes an RNA-binding protein that is involved in growth regulation. This protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing. This protein can interact with the cytoplasmic domain of the ErbB3 receptor and may contribute to transducing growth regulatory signals. This protein is also a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases. This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells. Six pseudogenes, located on chromosomes 3, 6, 9, 18, 20 and X, have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Ebp1: May play a role in a ERBB3-regulated signal transduction pathway. Seems be involved in growth regulation. Acts a corepressor of the androgen receptor (AR) and is regulated by the ERBB3 ligand neuregulin-1/heregulin (HRG). Inhibits transcription of some E2F1-regulated promoters, probably by recruiting histone acetylase (HAT) activity. Binds RNA. Associates with 28S, 18S and 5.8S mature rRNAs, several rRNA precursors and probably U3 small nucleolar RNA. May be involved in regulation of intermediate and late steps of rRNA processing. May be involved in ribosome assembly. Mediates cap-independent translation of specific viral IRESs (internal ribosomal entry site). Belongs to the peptidase M24 family.

Protein type: Nucleolus; Transcription, coactivator/corepressor; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 12q13.2

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nucleus; ribonucleoprotein complex

Molecular Function: protein binding; DNA binding; ubiquitin protein ligase binding; transcription factor activity

Biological Process: regulation of translation; cell proliferation; transcription, DNA-dependent; positive regulation of cell differentiation; negative regulation of transcription, DNA-dependent; cell cycle arrest; rRNA processing; negative regulation of apoptosis