Western Blot (WB)
(Western blot analysis of extracts from A375Â , using EAAT4 Antibody. The lane on the left was treated with blocking peptide.)
Rabbit EAAT4 Polyclonal Antibody | anti-SLC1A6 antibody
EAAT4 Antibody
Gene Names
SLC1A6; EAAT4
Reactivity
Human, Mouse, RatPredicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%)
Applications
ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
EAAT4; Polyclonal Antibody; EAAT4 Antibody; anti-SLC1A6 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%)
Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%)
Clonality
Polyclonal
Isotype
IgG
Specificity
EAAT4 Antibody detects endogenous levels of total EAAT4.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
Concentration
1mg/ml (varies by lot)
Sequence Length
312
Applicable Applications for anti-SLC1A6 antibody
ELISA (EIA)
Application Notes
WB: 1:500-1:2000
ELISA(peptide): 1:20,000-1:40,000
ELISA(peptide): 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human EAAT4, corresponding to a region within C-terminal amino acids.
Fragment
Fab fragment
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from A375Â , using EAAT4 Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from A375Â , using EAAT4 Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-SLC1A6 antibody
Function: Sodium-dependent, high-affinity amino acid transporter that mediates the uptake of L-glutamate and also L-aspartate and D-aspartate (PubMed:7791878). Functions as a symporter that transports one amino acid molecule together with two or three Na(+) ions and one proton, in parallel with the counter-transport of one K(+) ion. Mediates Cl(-) flux that is not coupled to amino acid transport; this avoids the accumulation of negative charges due to aspartate and Na(+) symport (By similarity). Plays a redundant role in the rapid removal of released glutamate from the synaptic cleft, which is essential for terminating the postsynaptic action of glutamate (Probable).
Subcellular Location: Cell membrane>Multi-pass membrane protein.
Tissue Specificity: Brain. Expressed densely and selectively in cell bodies of Purkinje cells.
Subunit Structure: Homotrimer.
Similarity: Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move.Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A6 subfamily.
Subcellular Location: Cell membrane>Multi-pass membrane protein.
Tissue Specificity: Brain. Expressed densely and selectively in cell bodies of Purkinje cells.
Subunit Structure: Homotrimer.
Similarity: Contains eight transmembrane regions plus two helical hairpins that dip into the membrane. These helical hairpin structures play an important role in the transport process. The first enters the membrane from the cytoplasmic side, the second one from the extracellular side. During the transport cycle, the regions involved in amino acid transport, and especially the helical hairpins, move vertically by about 15-18 Angstroms, alternating between exposure to the aqueous phase and reinsertion in the lipid bilayer. In contrast, the regions involved in trimerization do not move.Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A6 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed Molecular Weight: (Observed)61kD.
Predicted Molecular Weight: (Calculated)62kDa.
Predicted Molecular Weight: (Calculated)62kDa.
NCBI Official Full Name
excitatory amino acid transporter 4 isoform 1
NCBI Official Synonym Full Names
solute carrier family 1 member 6
NCBI Official Symbol
SLC1A6
NCBI Official Synonym Symbols
EAAT4
NCBI Protein Information
excitatory amino acid transporter 4
UniProt Protein Name
Excitatory amino acid transporter 4
Protein Family
UniProt Gene Name
SLC1A6
UniProt Synonym Gene Names
EAAT4
UniProt Entry Name
EAA4_HUMAN
Uniprot Description
SLC1A6: Transports L-glutamate and also L- and D-aspartate. Seems to act as a symport by cotransporting sodium. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A6 subfamily.
Protein type: Transporter; Membrane protein, integral; Transporter, SLC family; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 19p13.12
Cellular Component: Golgi apparatus; membrane; integral to plasma membrane; plasma membrane
Molecular Function: L-aspartate transmembrane transporter activity; sodium:dicarboxylate symporter activity; L-glutamate transmembrane transporter activity
Biological Process: synaptic transmission; regulation of membrane potential; L-glutamate transport; ion transport; aspartate transport; transmembrane transport
Research Articles on SLC1A6
Similar Products
Product Notes
The SLC1A6 slc1a6 (Catalog #AAA9612353) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EAAT4 Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's EAAT4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the SLC1A6 slc1a6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EAAT4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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