Western Blot (WB)
(All lanes: E3 Ribonucleoside-diphosphate reductase subunit M2 B antibody at 6ug/mlLane 1:MCF-7 whole cell lysateLane 2:Hela whole cell lysateLane 3:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 41,35,16,8,5,49 kDaObserved band size: 41 kDa)
Rabbit anti-Human E3 Ribonucleoside-diphosphate reductase subunit M2 B Polyclonal Antibody | anti-RRM2B antibody
Rabbit anti-human E3 Ribonucleoside-diphosphate reductase subunit M2 B polyclonal Antibody(RRM2B)
IHC:1:20-1:200
Western Blot (WB)
(All lanes: E3 Ribonucleoside-diphosphate reductase subunit M2 B antibody at 6ug/mlLane 1:MCF-7 whole cell lysateLane 2:Hela whole cell lysateLane 3:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 41,35,16,8,5,49 kDaObserved band size: 41 kDa)
Western Blot (WB)
(All lanes: E3 Ribonucleoside-diphosphate reductase subunit M2 B antibody at 6ug/mlLane 1:MCF-7 whole cell lysateLane 2:Hela whole cell lysateLane 3:HepG2 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 41,35,16,8,5,49 kDaObserved band size: 41 kDa)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung using MBS1499914 at dilution 1:100)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human lung using MBS1499914 at dilution 1:100)
Immunoprecipitation (IP)
(Immunoprecipitating RRM2B in MCF-7 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1499914 in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1499914(4ug)+ MCF-7 whole cell lysate(500ug)Lane 3: MCF-7 whole cell lysate (20ug))
Immunoprecipitation (IP)
(Immunoprecipitating RRM2B in MCF-7 whole cell lysateLane 1: Rabbit monoclonal IgG(1ug)instead of MBS1499914 in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the Secondary antibody (1/50000)Lane 2: MBS1499914(4ug)+ MCF-7 whole cell lysate(500ug)Lane 3: MCF-7 whole cell lysate (20ug))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the small subunit of a p53-inducible ribonucleotide reductase. This heterotetrameric enzyme catalyzes the conversion of ribonucleoside diphosphates to deoxyribonucleoside diphosphates. The product of this reaction is necessary for DNA synthesis. Mutations in this gene have been associated with autosomal recessive mitochondrial DNA depletion syndrome, autosomal dominant progressive external ophthalmoplegia-5, and mitochondrial neurogastrointestinal encephalopathy. Alternatively spliced transcript variants have been described.[provided by RefSeq, Feb 2010]
Uniprot Description
Plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. Supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. Contains an iron-tyrosyl free radical center required for catalysis. Forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage.
Research Articles on RRM2B
Similar Products
Product Notes
The RRM2B rrm2b (Catalog #AAA1499914) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human E3 Ribonucleoside-diphosphate reductase subunit M2 B polyclonal Antibody(RRM2B) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's E3 Ribonucleoside-diphosphate reductase subunit M2 B can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). Recommended dilution: WB:1:500-2000 IHC:1:20-1:200. Researchers should empirically determine the suitability of the RRM2B rrm2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E3 Ribonucleoside-diphosphate reductase subunit M2 B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
