Chicken E2IG3 Polyclonal Antibody | anti-GNL3 antibody

E2IG3 (Nucleostemin)

Cat. Num. AAA614240

• Gene Names: GNL3; NS; E2IG3; C77032; MGC800
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Purified; Purified.
• Synonyms: E2IG3; Polyclonal Antibody; E2IG3 (Nucleostemin); Anti -E2IG3 (Nucleostemin); anti-GNL3 antibody

• Host: Chicken
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes human E2IG3. Species Crossreactivity: mouse and rat.
• Purity/Purification: Purified; Purified.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, before the addition of glycerol to 40%.

• Applicable Applications for anti-GNL3 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.
• Immunogen: E2IG3 (Nucleostemin) aa1-280.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-GNL3 antibody

Nucleostemin; putative nucleotide binding protein, estradiol-induced; E2IG3. This protein may play a role in maintaining stem cell self-renewal. Depletion or overexpression of nucleostemin reduces cell proliferation in CNS stem cells and transformed cells. Mutation analysis indicated that excessive nucleostemin, particularly mutants lacking the GTP-regulatory domain, prevents cells from entering mitosis and causes apoptosis in a p53-dependent manner. The N-terminal basic domain mediates the interaction with p53 and is required for the cell death caused by overexpression.

Product Categories/Family for anti-GNL3 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 6457340
• NCBI GeneID: 26354
• UniProt Accession #: Q9BVP2; Q5PU80; Q96SV6; Q96SV7; Q9UJY0; B2RDC1
• Molecular Weight: 61,993 Da
• NCBI Official Full Name: E2IG3
• NCBI Official Synonym Full Names: guanine nucleotide binding protein-like 3 (nucleolar)
• NCBI Official Symbol: GNL3
• NCBI Official Synonym Symbols: NS; E2IG3; C77032; MGC800
• NCBI Protein Information: guanine nucleotide-binding protein-like 3; NNP47; nucleostemin; OTTHUMP00000213382; OTTHUMP00000213383; E2-induced gene 3 protein; novel nucleolar protein 47; nucleolar GTP-binding protein 3; estradiol-induced nucleotide binding protein
• UniProt Protein Name: Guanine nucleotide-binding protein-like 3
• Protein Family: Guanine nucleotide-binding protein
• UniProt Gene Name: GNL3
• UniProt Synonym Gene Names: E2IG3; NS
• UniProt Entry Name: GNL3_HUMAN

NCBI Description

The protein encoded by this gene may interact with p53 and may be involved in tumorigenesis. The encoded protein also appears to be important for stem cell proliferation. This protein is found in both the nucleus and nucleolus. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

GNL3: May be required to maintain the proliferative capacity of stem cells and may play an important role in tumorigenesis. May interact with p53/TP53 via its basic domain. Increased levels in lung tissue in cancer patients. Belongs to the MMR1/HSR1 GTP-binding protein family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Nucleolus; RNA-binding; Cell cycle regulation

Chromosomal Location of Human Ortholog: 3p21.1

Cellular Component: nucleoplasm; extracellular space; membrane; nucleolus; nucleus

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: cell proliferation; metabolic process; ribosome biogenesis and assembly; regulation of cell proliferation