Rabbit anti-Human E2F5 Polyclonal Antibody | anti-E2F5 antibody

E2F5, NT (Transcription Factor E2F5, E2F-5) (AP)

Cat. Num. AAA6473397

• Gene Names: E2F5; E2F-5
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: E2F5; Polyclonal Antibody; NT (Transcription Factor E2F5; E2F-5) (AP); anti-E2F5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human E2F5.
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-E2F5 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-1:500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human E2F5 (KLH).
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of E2F5 Antibody (N-term) in HepG2 cell line lysates (35ug/lane). E2F5 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with E2F5 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(E2F5 Antibody (N-term) FC analysis of ZR-75-1 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-E2F5 antibody

Antibodies; Transcription Factors

References

1. Itoh, A., et.al., Cell. Mol. Biol. Res. 41(3), 147-154 (1995).

NCBI and Uniprot Product Information

• NCBI GI #: 134142811
• NCBI GeneID: 1875
• NCBI Accession #: NP_001942.2
• NCBI GenBank Nucleotide #: NM_001951.3
• UniProt Accession #: Q15329; Q16601; Q92756; E9PBN9
• Molecular Weight: 20,192 Da
• NCBI Official Full Name: transcription factor E2F5 isoform 1
• NCBI Official Synonym Full Names: E2F transcription factor 5, p130-binding
• NCBI Official Symbol: E2F5
• NCBI Official Synonym Symbols: E2F-5
• NCBI Protein Information: transcription factor E2F5
• UniProt Protein Name: Transcription factor E2F5
• Protein Family: Transcription factor
• UniProt Gene Name: E2F5
• UniProt Synonym Gene Names: E2F-5
• UniProt Entry Name: E2F5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F5: a member of the E2F/DP family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Differentially phosphorylated and is expressed in a wide variety of human tissues. It is more homologous to E2F4 than to other E2F/DP family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleolus; nucleus

Molecular Function: protein binding; DNA binding; transcription factor activity; transcription factor binding

Biological Process: transcription initiation from RNA polymerase II promoter; organ morphogenesis; transcription, DNA-dependent; cell projection organization and biogenesis; regulation of cell cycle; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle