Goat E. coli Polyclonal Antibody
E. coli
Applications
Immunofluorescence
Synonyms
E. coli; Polyclonal Antibody; E. coli Antiserum Products; anti-E. coli antibody
Host
Goat
Clonality
Polyclonal
Specificity
Many "O" and "K" antigenic serotypes of Escherichia coli
Form/Format
FITC
Applicable Applications for anti-E. coli antibody
Immunofluorescence
Application Notes
Titer: > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12
Immunogen
Mixture of E. coli serotypes
Reactivity Note
Antiserum is unabsorbed and does crossreact with related Enterobacteriaceae
Antiserum Characterization
(neat)
Related Product Information for anti-E. coli antibody
This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product. The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/ml) as a stabilizer and 0.1% sodium azide preservative. Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. ViroStat also recommends use of its COUNTERSTAIN/BLOCKING DILUENT (Cat.#4400) and its MOUNTINGMEDIUM (Cat.#4300) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing ViroStat's enzyme-antibody conjugates specific to FITC (Cat.#s 3104 & 3105). Recommened short term (<6 months) storage is liquid at 2-8 degree C under subdued light. For longer term storage, aliquot and freeze. Immunoglobulin concentration is 4-5 mg/ml. Unit size is 1.0 ml.
Product Categories/Family for anti-E. coli antibody
Similar Products
Product Notes
The E. coli (Catalog #AAA324062) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's E. coli can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence. Titer: > 1:1,600 by indirect IFA vs serotypes O157:H7, O20, O125, O55, O111 & K12. Researchers should empirically determine the suitability of the E. coli for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "E. coli, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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