Western Blot (WB)
(Western blot analysis of DYRK1A/B (Phospho-Tyr321/273) using EGF treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit DYRK1A/B Polyclonal Antibody | anti-DYRK1A/B antibody
Phospho-DYRK1A/B (Tyr321/Tyr273) Antibody
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%)
Tissue Specificity: Highest expression in skeletal muscle, testis, heart and brain with little expression in colon or lung. Expressed in a variety of tumor cell lines.
IHC: 1:50-1:200
Peptide ELISA: 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of DYRK1A/B (Phospho-Tyr321/273) using EGF treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis of DYRK1A/B (Phospho-Tyr321/273) using EGF treated K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Immunohistochemistry (IHC)
(Staining Human normal tissues adjacent to pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(Staining Human normal tissues adjacent to pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Mouse kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)
(At 1/200 staining Human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Dual-specificity tyrosine phosphorylation-regulated kinase (DYRK) family. This member contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. It catalyzes its autophosphorylation on serine/threonine and tyrosine residues. It may play a significant role in a signaling pathway regulating cell proliferation and may be involved in brain development. This gene is a homolog of Drosophila mnb (minibrain) gene and rat Dyrk gene. It is localized in the Down syndrome critical region of chromosome 21, and is considered to be a strong candidate gene for learning defects associated with Down syndrome. Alternative splicing of this gene generates several transcript variants differing from each other either in the 5' UTR or in the 3' coding region. These variants encode at least five different isoforms. [provided by RefSeq, Jul 2008]
Uniprot Description
DYRK1A: a dual-specificity protein kinase of the DYRK family. Contains a nuclear targeting signal sequence, a protein kinase domain, a leucine zipper motif, and a highly conservative 13-consecutive-histidine repeat. May play a significant role in a pathway regulating cell proliferation and may be involved in brain development. Its gene is localized in the Down syndrome (DS) critical region of chromosome 21, and is a strong candidate gene for learning defects associated with Down syndrome. Overexpression in mice gives rise to neurological abnormalities similar to those observed in DS. Five splice-variant isoforms have been described.
Protein type: Protein kinase, CMGC; EC 2.7.12.2; Protein kinase, dual-specificity (non-receptor); Kinase, protein; CMGC group; DYRK family; Dyrk1 subfamily
Chromosomal Location of Human Ortholog: 21q22.13
Cellular Component: nucleoplasm; nuclear speck; ribonucleoprotein complex; nucleus
Molecular Function: protein serine/threonine kinase activity; identical protein binding; protein binding; protein self-association; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; protein serine/threonine/tyrosine kinase activity; tau protein binding; ATP binding; protein kinase activity
Biological Process: circadian rhythm; nervous system development; negative regulation of DNA damage response, signal transduction by p53 class mediator; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation; mitotic cell cycle; protein amino acid phosphorylation; regulation of alternative nuclear mRNA splicing, via spliceosome
Disease: Mental Retardation, Autosomal Dominant 7
Research Articles on DYRK1A/B
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Product Notes
The DYRK1A/B dyrk1a (Catalog #AAA9614142) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-DYRK1A/B (Tyr321/Tyr273) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's DYRK1A/B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA). WB: 1:1000-3000 IHC: 1:50-1:200 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the DYRK1A/B dyrk1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DYRK1A/B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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