Rabbit DYNORPHIN A Polyclonal Antibody | anti-PDYN antibody

RABBIT ANTI HUMAN DYNORPHIN A (aa1-17)

Cat. Num. AAA222374

• Gene Names: PDYN; ADCA; PENKB; SCA23
• Reactivity: Pig, Rat, Rhesus Monkey
• Applications: Immunohistochemistry, Immunohistochemistry, immunoassay, Radioimmunoassay
• Synonyms: DYNORPHIN A; Polyclonal Antibody; RABBIT ANTI HUMAN DYNORPHIN A (aa1-17); anti-PDYN antibody

• Host: Rabbit
• Reactivity: Pig, Rat, Rhesus Monkey
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Serum; Serum - liquid
• Sequence Length: 254

• Applicable Applications for anti-PDYN antibody: Immunohistology Frozen, Immunohistology Paraffin*, Radioimmunoassays (RIA)
• Application Notes: Immunohistology - Frozen: Minimum Dilution: 1/100; Maximum Dilution: 1/1500;; Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; ; ; Overnight incubation is recommended for paraffin sections.; Radioimmunoassays: Maximum Dilution: 1/10,000
• Perservative Stabilisers: 0.09% Sodium Azide; 0.1% Bovine Serum Albumin
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to Dynorphin A 1-17 were raised by repeated immunisation of rabbits with highly purified antigen. Whole serum is supplied - diluted.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-PDYN antibody

Rabbit anti Human dynorphin A antibody recognizes the opioid peptide dynorphin A (Fischli et al. 1982), a cleavage product of big dynorphin, itself derived from the precursor pro-encephalin-B, also known as preprodynorphin. Rabbit anti human dynorphin A antibody binds to both dynorphin A 1-17 and 1-8 but not with other big dynorphin cleavage products such as dynorphin B, also known as rimorphin. Dynorphin A immunoreactive cells are found in the paraventricular and supraoptic nucleus and in hippocampal mossy fibers of rat brain following kainic acid administration and colchicine induced neurotoxicity (McGinty et al. 1983). Dynorphins play a role in multiple physiologic processes including pain perception (Wang et al. 2001)and responses to stress (Rácz et al. 2013). They mimic and compete with a number of opiate drugs(Chavkin 2013). Dysregulation of dynorphin A expression has been linked to progression of neurodegenerative conditions such as Altzheimer's disease (Yakovleva et al. 2007) and appears to play a significant role in the pathobiology of epilepsy (Loacker et al. 2007). Rabbit anti human dynorphin antibody has been used successfully for the immunohistochemical demonstration of ˜mossy fibre' sprouting in hippocampal sclerosis (Thom et al. 2009) and in a virally induced disease model in the rat (Solbrig et al. 2006).

NCBI and Uniprot Product Information

• NCBI GI #: 300116201
• NCBI GeneID: 5173
• NCBI Accession #: NP_001177821.1
• NCBI GenBank Nucleotide #: NM_001190892.1
• UniProt Accession #: P01213; A8K0Q3
• Molecular Weight: 28,385 Da
• NCBI Official Full Name: proenkephalin-B preproprotein
• NCBI Official Synonym Full Names: prodynorphin
• NCBI Official Symbol: PDYN
• NCBI Official Synonym Symbols: ADCA; PENKB; SCA23
• NCBI Protein Information: proenkephalin-B; beta-neoendorphin-dynorphin; leu-enkephalin; leumorphin; neoendorphin-dynorphin-enkephalin prepropeptide; preprodynorphin; preproenkephalin B; rimorphin
• UniProt Protein Name: Proenkephalin-B
• Protein Family: Proenkephalin
• UniProt Gene Name: PDYN
• UniProt Synonym Gene Names: Big Dyn; Dyn-A17; Dynorphin A; Dyn-B
• UniProt Entry Name: PDYN_HUMAN

NCBI Description

The protein encoded by this gene is a preproprotein that is proteolytically processed to form the secreted opioid peptides beta-neoendorphin, dynorphin, leu-enkephalin, rimorphin, and leumorphin. These peptides are ligands for the kappa-type of opioid receptor. Dynorphin is involved in modulating responses to several psychoactive substances, including cocaine. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

PDYN: Leu-enkephalins compete with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress. Defects in PDYN are the cause of spinocerebellar ataxia type 23 (SCA23). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA23 is an adult-onset autosomal dominant form characterized by slowly progressive gait and limb ataxia, with variable additional features, including peripheral neuropathy and dysarthria. Belongs to the opioid neuropeptide precursor family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: extracellular region; plasma membrane

Molecular Function: opioid peptide activity

Biological Process: synaptic transmission; neuropeptide signaling pathway

Disease: Spinocerebellar Ataxia 23