Rabbit Dynorphin A, aa1-17 Polyclonal Antibody | anti-DNM1 antibody

Dynorphin A, aa1-17

Cat. Num. AAA646525

• Gene Names: DNM1; DNM
• Reactivity: Human, Monkey, Porcine, Rat
• Applications: Immunohistochemistry, immunoassay, Radioimmunoassay
• Purity: Serum; Serum
• Synonyms: Dynorphin A; aa1-17; Polyclonal Antibody; Anti -Dynorphin A; anti-DNM1 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Porcine, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes porcine Dynorphin A. Blocks with dynorphin 1-17 and 1-8. Does not block other related peptides. Cells are found in the paraventricular and supraoptic nucleus. Fibers are found in the lateral hypothalamus in rat brain of 4% FA perfused colchicine tissue. Species Crossreactivity: monkey, rat, Rhesus monkey and human.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide, 0.1% BSA before the addition of glycerol to 40%.

• Applicable Applications for anti-DNM1 antibody: Immunohistochemistry (IHC), Radioimmunoassay (RIA)
• Application Notes: Suitable for use in Immunohistochemistry and Radioimmunoassays.; Dilution: Immunohistochemistry (frozen): 1:50-1:1250; Immunohistochemistry: Paraffin sections require antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer, pH 6.0 is recommended. Overnight incubation is recommended.; Radioimmunoassay: 1:15000; Optimal working dilutions to be determined by researcher.
• Immunogen: Synthetic peptide, Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ile-Arg-Pro-Lys-Leu- Lys-Trp-Asp-Asn-Gln corresponding to aa1-17 of porcine Dynorphin A.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-DNM1 antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 59853099
• NCBI GeneID: 1759
• NCBI Accession #: NP_004399.2
• NCBI GenBank Nucleotide #: NM_004408.2
• UniProt Accession #: Q05193; Q5SYX0; Q5SYX2; Q6P3T6; Q86VD2; A6NLM6
• Molecular Weight: 97,408 Da
• NCBI Official Full Name: dynamin-1 isoform 1
• NCBI Official Synonym Full Names: dynamin 1
• NCBI Official Symbol: DNM1
• NCBI Official Synonym Symbols: DNM
• NCBI Protein Information: dynamin-1
• UniProt Protein Name: Dynamin-1
• Protein Family: Dynamin
• UniProt Gene Name: DNM1
• UniProt Synonym Gene Names: DNM
• UniProt Entry Name: DYN1_HUMAN

NCBI Description

This gene encodes a member of the dynamin subfamily of GTP-binding proteins. The encoded protein possesses unique mechanochemical properties used to tubulate and sever membranes, and is involved in clathrin-mediated endocytosis and other vesicular trafficking processes. Actin and other cytoskeletal proteins act as binding partners for the encoded protein, which can also self-assemble leading to stimulation of GTPase activity. More than sixty highly conserved copies of the 3' region of this gene are found elsewhere in the genome, particularly on chromosomes Y and 15. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes. Involved in receptor-mediated endocytosis.

Catalytic activity: GTP + H2O = GDP + phosphate.

Subunit structure: Interacts with CAV1 and SH3GLB1. Binds SH3GL1, SH3GL2 and SH3GL3

By similarity. Interacts with PHOCN. Interacts with PACSIN1, PACSIN2 and PACSIN3

By similarity. Interacts with SNX9. Interacts with MYO1E (via SH3 domain). Interacts with SNX33 (via SH3 domain). Ref.4 Ref.6 Ref.7

Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton. Note: Microtubule-associated. Ref.4

Sequence similarities: Belongs to the dynamin family.Contains 1 GED domain.Contains 1 PH domain.