Western Blot (WB)
(Lane A - (1ug/ml) staining of HEK293 overexpressing Human DYDC1 lysate (10ug protein in RIPA buffer) Lane B - (1ug/ml) staining of HEK293 mock-transfected lysate (10ug protein in RIPA buffer). Lane C - anti-MYC Tag (1/1000) staining HEK293 overexpressing Human DYDC1 lysate (10ug protein in RIPA buffer). Primary incubations were for 1 hour. Detected by chemiluminescence.)
Goat DYDC1 Polyclonal Antibody | anti-DYDC1 antibody
Goat anti-DYDC1 Antibody
Gene Names
DYDC1; DPY30D1
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
DYDC1; Polyclonal Antibody; Goat anti-DYDC1 Antibody; Goat Anti-DYDC1 (aa142-154) Antibody; DPY30 domain containing 1; DPY30D1; FLJ43920; bA36D19.5; BC019250 protein; OTTHUMP00000019963; bA36D19.5 antibody; BC019250 protein antibody; DPY30 domain containing 1 antibody; DPY30D1 antibody; FLJ43920 antibody; OTTHUMP00000019963 antibody; DYDC1 antibody; anti-DYDC1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
RNEDILHSEEATLD
Sequence Length
177
Applicable Applications for anti-DYDC1 antibody
Peptide ELISA (EIA), Western Blot (WB-Trf)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: In transfected HEK293 transiently expressing Human DYDC1 (myc and DYKDDDDK tagged), a band of approx.28kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1ug/ml.
Western Blot: In transfected HEK293 transiently expressing Human DYDC1 (myc and DYKDDDDK tagged), a band of approx.28kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1ug/ml.
Immunogen
Peptide with sequence C-RNEDILHSEEATLD, from the internal region of the protein sequence according to NP_620167.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
(Lane A - (1ug/ml) staining of HEK293 overexpressing Human DYDC1 lysate (10ug protein in RIPA buffer) Lane B - (1ug/ml) staining of HEK293 mock-transfected lysate (10ug protein in RIPA buffer). Lane C - anti-MYC Tag (1/1000) staining HEK293 overexpressing Human DYDC1 lysate (10ug protein in RIPA buffer). Primary incubations were for 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(Lane A - (1ug/ml) staining of HEK293 overexpressing Human DYDC1 lysate (10ug protein in RIPA buffer) Lane B - (1ug/ml) staining of HEK293 mock-transfected lysate (10ug protein in RIPA buffer). Lane C - anti-MYC Tag (1/1000) staining HEK293 overexpressing Human DYDC1 lysate (10ug protein in RIPA buffer). Primary incubations were for 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
21,795 Da
NCBI Official Full Name
DPY30 domain-containing protein 1
NCBI Official Synonym Full Names
DPY30 domain containing 1
NCBI Official Symbol
DYDC1
NCBI Official Synonym Symbols
DPY30D1
NCBI Protein Information
DPY30 domain-containing protein 1
UniProt Protein Name
DPY30 domain-containing protein 1
Protein Family
UniProt Gene Name
DYDC1
UniProt Synonym Gene Names
DPY30D1
UniProt Entry Name
DYDC1_HUMAN
NCBI Description
This gene encodes a member of a family of proteins that contains a DPY30 domain. The encoded protein is involved in acrosome formation during spermatid development. This gene locus overlaps with a closely related gene on the opposite strand. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]
Uniprot Description
DYDC1: Plays a crucial role during acrosome biogenesis. Belongs to the dpy-30 family.
Chromosomal Location of Human Ortholog: 10q23.1
Cellular Component: chromosome, telomeric region
Molecular Function: histone lysine N-methyltransferase activity (H3-K4 specific); protein binding
Biological Process: chromatin silencing at telomere; histone H3-K4 methylation
Research Articles on DYDC1
Similar Products
Product Notes
The DYDC1 dydc1 (Catalog #AAA421856) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DYDC1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's DYDC1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB-Trf). Peptide ELISA: Antibody detection limit dilution 1: 16000. Western Blot: In transfected HEK293 transiently expressing Human DYDC1 (myc and DYKDDDDK tagged), a band of approx.28kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc antibody. Recommended concentration, 0.5-1ug/ml. Researchers should empirically determine the suitability of the DYDC1 dydc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: RNEDILHSEE ATLD. It is sometimes possible for the material contained within the vial of "DYDC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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