Rabbit anti-Human DUT Polyclonal Antibody | anti-DUT antibody

DUT, CT (DUT, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial, dUTP pyrophosphatase) (AP)

Cat. Num. AAA6293851

• Gene Names: DUT; dUTPase
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DUT; Polyclonal Antibody; CT (DUT; Deoxyuridine 5'-triphosphate nucleotidohydrolase; mitochondrial; dUTP pyrophosphatase) (AP); dUTP pyrophosphatase; anti-DUT antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-DUT antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DUT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 169-198 amino acids from the C-terminal region of human DUT.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DUT Antibody (C-term) western blot analysis in human normal Uterus tissue lysates (35ug/lane).This demonstrates the DUT antibody detected the DUT protein (arrow).)

Immunohistochemistry (IHC)

(DUT Antibody (C-term) (AP14203b)immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DUT Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-DUT antibody

Antibodies; Mitochondrial Proteins

References

Takatori, H., et al. Liver Int. 30(3):438-446(2010). Quesada-Soriano, I., et al. Biochimie 92(2):178-186(2010). Chanson, A., et al. Am. J. Clin. Nutr. 89(6):1927-1936(2009). Takacs, E., et al. FEBS Lett. 583(5):865-871(2009). Venkatesan, K., et al. Nat. Methods 6(1):83-90(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4503423
• NCBI GeneID: 1854
• NCBI Accession #: NP_001939.1; NP_001020419.1; NP_001020420.1
• NCBI GenBank Nucleotide #: NM_001948.3
• UniProt Accession #: P33316; O14785; Q16708; Q16860; Q6FHN1; Q6NSA3; Q96Q81; A8K650; B4DPR5
• Molecular Weight: 17,748 Da
• NCBI Official Full Name: deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial isoform 2
• NCBI Official Synonym Full Names: deoxyuridine triphosphatase
• NCBI Official Symbol: DUT
• NCBI Official Synonym Symbols: dUTPase
• NCBI Protein Information: deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial; dUTP pyrophosphatase; dUTP nucleotidohydrolase
• UniProt Protein Name: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
• Protein Family: Deoxyuridine 5'-triphosphate nucleotidohydrolase
• UniProt Gene Name: DUT
• UniProt Synonym Gene Names: dUTPase
• UniProt Entry Name: DUT_HUMAN

NCBI Description

This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. [provided by RefSeq, Jul 2008]

Uniprot Description

dUTPase: This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. Belongs to the dUTPase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; Motility/polarity/chemotaxis; Mitochondrial; DNA replication; Nuclear receptor co-regulator; Nucleotide Metabolism - pyrimidine; EC 3.6.1.23

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: nucleoplasm; mitochondrion; nucleus

Molecular Function: protein binding; magnesium ion binding; dUTP diphosphatase activity

Biological Process: dUTP catabolic process; pyrimidine base metabolic process; nucleobase, nucleoside and nucleotide metabolic process; pyrimidine nucleoside biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; DNA replication; dUMP biosynthetic process