Rabbit anti-Human DUSP14 Polyclonal Antibody | anti-DUSP14 antibody

DUSP14, ID (DUSP14, MKP6, Dual specificity protein phosphatase 14, MKP-1-like protein tyrosine phosphatase, Mitogen-activated protein kinase phosphatase 6) (Biotin)

Cat. Num. AAA6293798

• Gene Names: DUSP14; MKP6; MKP-L
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DUSP14; Polyclonal Antibody; ID (DUSP14; MKP6; Dual specificity protein phosphatase 14; MKP-1-like protein tyrosine phosphatase; Mitogen-activated protein kinase phosphatase 6) (Biotin); Mitogen-activated protein kinase phosphatase 6; anti-DUSP14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-DUSP14 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DUSP14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 76-105 amino acids from the Central region of human DUSP14.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DUSP14 Antibody (Center) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DUSP14 antibody detected the DUSP14 protein (arrow).)

Related Product Information for anti-DUSP14 antibody

Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. They have been implicated as major modulators of critical signaling pathways. DUSP14 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs (see MIM 600714) (summary by Patterson et al., 2009 [PubMed 19228121]).

Product Categories/Family for anti-DUSP14 antibody

Antibodies; Protein Kinases

References

Thye, T., et al. Nat. Genet. 42(9):739-741(2010). Lountos, G.T., et al. Acta Crystallogr. D Biol. Crystallogr. 65 (PT 10), 1013-1020 (2009): Patterson, K.I., et al. Biochem. J. 418(3):475-489(2009). Elass, E., et al. FEBS Lett. 582(3):445-450(2008). Nyati, M.K., et al. Cancer Res. 66(24):11554-11559(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 5902002
• NCBI GeneID: 11072
• NCBI Accession #: NP_008957.1
• NCBI GenBank Nucleotide #: NM_007026.3
• UniProt Accession #: O95147
• Molecular Weight: 22,255 Da
• NCBI Official Full Name: dual specificity protein phosphatase 14
• NCBI Official Synonym Full Names: dual specificity phosphatase 14
• NCBI Official Symbol: DUSP14
• NCBI Official Synonym Symbols: MKP6; MKP-L
• NCBI Protein Information: dual specificity protein phosphatase 14; MKP-6; MAP kinase phosphatase 6; MKP-1 like protein tyrosine phosphatase; MKP-1-like protein tyrosine phosphatase; mitogen-activated protein kinase phosphatase 6
• UniProt Protein Name: Dual specificity protein phosphatase 14
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP14
• UniProt Synonym Gene Names: MKP6; MKP-L; MAP kinase phosphatase 6; MKP-6
• UniProt Entry Name: DUS14_HUMAN

NCBI Description

Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. They have been implicated as major modulators of critical signaling pathways. DUSP14 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs (see MIM 600714) (summary by Patterson et al., 2009 [PubMed 19228121]).[supplied by OMIM, Dec 2009]

Uniprot Description

MKP-6: a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (ERK, JNK, p38). Is a negative regulator of T cell costimulation via CD28.

Protein type: Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity; EC 3.1.3.48; EC 3.1.3.16

Chromosomal Location of Human Ortholog: 17q12

Molecular Function: protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; MAP kinase tyrosine/serine/threonine phosphatase activity

Biological Process: inactivation of MAPK activity