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Immunohistochemistry (IHC) (DUS1L Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DUS1L Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Rabbit anti-Human DUS1L Polyclonal Antibody | anti-DUS1L antibody

DUS1L, CT (DUS1L, tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like, tRNA-dihydrouridine synthase 1-like) (Biotin)

Gene Names
DUS1L; DUS1; PP3111
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DUS1L; Polyclonal Antibody; CT (DUS1L; tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like; tRNA-dihydrouridine synthase 1-like) (Biotin); tRNA-dihydrouridine synthase 1-like; anti-DUS1L antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Applicable Applications for anti-DUS1L antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
DUS1L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 320-349 amino acids from the C-terminal region of human DUS1L.
Conjugate
Biotin
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(DUS1L Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DUS1L Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (DUS1L Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DUS1L Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(DUS1L Antibody (C-term) western blot analysis in HepG2,NCI-H460,NCI-H292,WiDr,Hela,U251 cell line lysates (35ug/lane).This demonstrates the DUS1L antibody detected the DUS1L protein (arrow).)

Western Blot (WB) (DUS1L Antibody (C-term) western blot analysis in HepG2,NCI-H460,NCI-H292,WiDr,Hela,U251 cell line lysates (35ug/lane).This demonstrates the DUS1L antibody detected the DUS1L protein (arrow).)
Related Product Information for anti-DUS1L antibody
DUS1L catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs (By similarity).
Product Categories/Family for anti-DUS1L antibody
References
Gerhard, D.S., et al. Genome Res. 14 (10B), 2121-2127 (2004).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
53,230 Da
NCBI Official Full Name
tRNA-dihydrouridine(16/17) synthase
NCBI Official Synonym Full Names
dihydrouridine synthase 1-like (S. cerevisiae)
NCBI Official Symbol
DUS1L
NCBI Official Synonym Symbols
DUS1; PP3111
NCBI Protein Information
tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like; tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
UniProt Protein Name
tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
UniProt Gene Name
DUS1L
UniProt Entry Name
DUS1L_HUMAN

Uniprot Description

DUS1L: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Belongs to the dus family. Dus1 subfamily.

Protein type: EC 1.3.1.-; Oxidoreductase; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 17q25.3

Molecular Function: FAD binding; tRNA dihydrouridine synthase activity

Similar Products

Product Notes

The DUS1L dus1l (Catalog #AAA6293732) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DUS1L, CT (DUS1L, tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like, tRNA-dihydrouridine synthase 1-like) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DUS1L can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DUS1L dus1l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DUS1L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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