Rabbit anti-Mouse Duodenal Cytochrome b Polyclonal Antibody | anti-CYBRD1 antibody

Duodenal Cytochrome b (Dcytb)

Cat. Num. AAA615358

• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Duodenal Cytochrome b; Polyclonal Antibody; Duodenal Cytochrome b (Dcytb); Anti -Duodenal Cytochrome b (Dcytb); anti-CYBRD1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Duodenal Cytochrome b (Dcytb). No significant sequence homology exists with b558 or other reductases. Species Sequence Homology
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-CYBRD1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml (ECL).; ELISA: 0.5-1ug/ml. Control peptide can be used to coat ELISA plates at 1ug/ml.
• Immunogen: Synthetic peptide consisting of 20aa sequence located within the cytoplasmic C-terminus of mouse DCYTB (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CYBRD1 antibody

Iron is an essential element for a variety physiological process. A variety of proteins are involved in the transport of iron. Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1. These transporters are especially active in small intestine where most dietary iron absorption is conducted. Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III). Therefore, highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion. A number of ferric reductases have been identified in yeast, plants, and bacteria but the identity of such proteins remained unknown in the mammals. Most recently, a new gene called Dcytb (for duodenal cytochrome b) has been cloned and characterized in mouse duodenum that may function as mammalian ferric reductase. Dcytb (human 286aa, rat 290aa) is a 6-transmembrane domains protein with ~45% sequence identity with cytochrome b561 reductase. No sequence homology exists with plant and yeast Dcytb. The N-terminus of mouse Dcytb is almost identical with the rabbit's p30 protein called cytochrome b558. Putative biding sites for cytochrome b561 substrates (ascorbic acid and semidehydroascorbic acid) are partially conserved in Dcytb and b561. Unlike most proteins involved in iron metabolism, Dcytb gene lacks a definable iron-responsive element. Dcytb is highly expressed in the brush border membrane of duodenal entrecotes.

Product Categories/Family for anti-CYBRD1 antibody

Antibodies; Abs to Enzymes; Cytochrome

NCBI and Uniprot Product Information

• NCBI GI #: 47522364
• UniProt Accession #: Q53TN4; Q6KC16; Q6KC17; Q6P147; Q9H0Q8; Q9H5G5; B2RE79
• Molecular Weight: 31,641 Da
• NCBI Official Full Name: duodenal cytochrome b
• UniProt Protein Name: Cytochrome b reductase 1
• Protein Family: Cytochrome b reductase
• UniProt Gene Name: CYBRD1
• UniProt Synonym Gene Names: DCYTB; FRRS3
• UniProt Entry Name: CYBR1_HUMAN

NCBI Description

This gene is a member of the cytochrome b(561) family that encodes an iron-regulated protein. It highly expressed in the duodenal brush border membrane. It has ferric reductase activity and is believed to play a physiological role in dietary iron absorption. [provided by RefSeq, Jul 2008]

Uniprot Description

CYBRD1: Ferric-chelate reductase that reduces Fe(3+) to Fe(2+). Present at the brush border of duodenal enterocytes where it probably reduces dietary Fe(3+) thereby facilitating its transport into the mucosal cells. Uses ascorbate as electron donor. May be involved in extracellular ascorbate recycling in erythrocyte membranes. May also act as a ferrireductase in airway epithelial cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 1.-.-.-; Transporter, ion channel; Oxidoreductase; Transporter, iron; Motility/polarity/chemotaxis; Membrane protein, integral; Membrane protein, multi-pass; Transporter

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: brush border membrane; integral to membrane; plasma membrane

Molecular Function: oxidoreductase activity, oxidizing metal ions; metal ion binding; ferric-chelate reductase activity

Biological Process: cellular iron ion homeostasis; response to iron ion; transmembrane transport