Rabbit anti-Human, Mouse DTX1 Polyclonal Antibody | anti-DTX1 antibody

DTX1 Antibody

Cat. Num. AAA9630550

• Gene Names: DTX1; hDx-1
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: DTX1; Polyclonal Antibody; DTX1 Antibody; Deltex 1; E3 ubiquitin ligase; Deltex; Deltex homolog 1 (Drosophila); Deltex homolog 1; Deltex protein 1; Deltex-1; Deltex1; dtx1; DTX1_HUMAN; E3 ubiquitin protein ligase DTX1; E3 ubiquitin-protein ligase DTX1; FXI-T1; Fxit 1; Fxit1; hDTX1; hDx 1; mDTX1; Protein deltex 1; Protein deltex-1; anti-DTX1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: DTX1 Antibody detects endogenous levels of DTX1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-DTX1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human DTX1.
• Conjugation: Unconjugated
• Similarity: The WWE domains are thought to mediate some protein-protein interaction, and are frequently found in ubiquitin ligases. Belongs to the Deltex family.
• Post Translational Modifications: Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
• Subunit Structure: Homodimer. May form a heterodimer with other members of the Deltex family. Interacts with NOTCH1 via its N-terminal region and EIF3F, the interaction is required for NOTCH1 deubiquitination. Interacts with EP300. Forms a heterodimer with BBAP; the heterodimerization leading to an increase of in vitro ubiquitin ligase activity. Interacts with ITCH.
• Tissue Specificity: Widely expressed. Strongly expressed in blood vessel. Also expressed in embryonic nervous system, pancreas, lung, adrenal gland, digestive tube and muscles. Expressed in MZB cells and developing B- and T-cells.
• Function: Functions as a ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity (By similarity). Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T-cell development, suggesting that it can antagonize NOTCH1.
• Note: Predominantly cytoplasmic. Associates with endocytic vesicles. Partially nuclear.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-DTX1 antibody

Cytoplasm; Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 41352718
• NCBI GeneID: 1840
• NCBI Accession #: NP_004407.2
• NCBI GenBank Nucleotide #: NM_004416.2
• UniProt Accession #: Q86Y01; O60630; Q9BS04
• Molecular Weight: 67,368 Da
• NCBI Official Full Name: E3 ubiquitin-protein ligase DTX1
• NCBI Official Synonym Full Names: deltex 1, E3 ubiquitin ligase
• NCBI Official Symbol: DTX1
• NCBI Official Synonym Symbols: hDx-1
• NCBI Protein Information: E3 ubiquitin-protein ligase DTX1; deltex homolog 1; deltex1; hDTX1; protein deltex-1
• UniProt Protein Name: E3 ubiquitin-protein ligase DTX1
• Protein Family: Protein DETOXIFICATION
• UniProt Gene Name: DTX1
• UniProt Synonym Gene Names: Deltex1; hDTX1
• UniProt Entry Name: DTX1_HUMAN

NCBI Description

Studies in Drosophila have identified this gene as encoding a positive regulator of the Notch-signaling pathway. The human gene encodes a protein of unknown function; however, it may play a role in basic helix-loop-helix transcription factor activity. [provided by RefSeq, Jul 2008]

Uniprot Description

DTX1: Functions as an ubiquitin ligase protein in vivo, mediating ubiquitination and promoting degradation of MEKK1, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity. Regulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Mainly acts as a positive regulator of Notch, but it also acts as a negative regulator, depending on the developmental and cell context. Mediates the antineural activity of Notch, possibly by inhibiting the transcriptional activation mediated by MATCH1. Involved in neurogenesis, lymphogenesis and myogenesis, and may also be involved in MZB (Marginal zone B) cell differentiation. Promotes B-cell development at the expense of T- cell development, suggesting that it can antagonize NOTCH1. Belongs to the Deltex family.

Protein type: Ubiquitin conjugating system; EC 6.3.2.-; Ubiquitin ligase; Ligase

Chromosomal Location of Human Ortholog: 12q24.13

Cellular Component: cytoplasm; cytosol; nucleus

Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; transcription coactivator activity; Notch binding; SH3 domain binding; ligase activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of neuron differentiation; Notch signaling pathway; glial cell differentiation; cell surface receptor linked signal transduction; regulation of Notch signaling pathway; transcription, DNA-dependent; protein ubiquitination; negative regulation of T cell differentiation