Rabbit anti-Human DTNBP1 Polyclonal Antibody | anti-DTNBP1 antibody

DTNBP1, NT (DTNBP1, Dysbindin, Dysbindin-1, Dystrobrevin-binding protein 1, Hermansky-Pudlak syndrome 7 protein) (FITC)

Cat. Num. AAA6293645

• Gene Names: DTNBP1; SDY; DBND; HPS7; My031; BLOC1S8
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DTNBP1; Polyclonal Antibody; NT (DTNBP1; Dysbindin; Dysbindin-1; Dystrobrevin-binding protein 1; Hermansky-Pudlak syndrome 7 protein) (FITC); Hermansky-Pudlak syndrome 7 protein; anti-DTNBP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
• Sequence Length: 303

• Applicable Applications for anti-DTNBP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DTNBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9-38 amino acids from the N-terminal region of human DTNBP1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DTNBP1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the DTNBP1 antibody detected the DTNBP1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of DTNBP1 (arrow) using rabbit polyclonal DTNBP1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the DTNBP1 gene.)

Related Product Information for anti-DTNBP1 antibody

This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. A similar protein in mouse is a component of a protein complex termed biogenesis of lysosome-related organelles complex 1 (BLOC-1), and binds to alpha-and beta-dystrobrevins, which are components of the dystrophin-associated protein complex (DPC). Mutations in this gene are associated with Hermansky-Pudlak syndrome type 7. This gene may also be associated with schizophrenia. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

Product Categories/Family for anti-DTNBP1 antibody

Antibodies; Binding Proteins

References

Haukvik, U.K., et al. Prog. Neuropsychopharmacol. Biol. Psychiatry 34(7):1259-1265(2010). Voisey, J., et al. Eur. Psychiatry 25(6):314-319(2010). Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Hashimoto, R., et al. Nihon Shinkei Seishin Yakurigaku Zasshi 30(3):103-107(2010). Voisey, J., et al. Behav Brain Funct 6, 41 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 34304368
• NCBI GeneID: 84062
• NCBI Accession #: NP_898861.1; NP_115498.2
• NCBI GenBank Nucleotide #: NP_898861.1
• UniProt Accession #: Q96EV8
• NCBI Official Full Name: dysbindin isoform b
• NCBI Official Synonym Full Names: dystrobrevin binding protein 1
• NCBI Official Symbol: DTNBP1
• NCBI Official Synonym Symbols: SDY; DBND; HPS7; My031; BLOC1S8
• NCBI Protein Information: dysbindin
• UniProt Protein Name: Dysbindin
• Protein Family: Dysbindin
• UniProt Gene Name: DTNBP1
• UniProt Synonym Gene Names: BLOC1S8; BLOC-1 subunit 8; HPS7 protein
• UniProt Entry Name: DTBP1_HUMAN

NCBI Description

This gene encodes a protein that may play a role in organelle biogenesis associated with melanosomes, platelet dense granules, and lysosomes. A similar protein in mouse is a component of a protein complex termed biogenesis of lysosome-related organelles complex 1 (BLOC-1), and binds to alpha- and beta-dystrobrevins, which are components of the dystrophin-associated protein complex (DPC). Mutations in this gene are associated with Hermansky-Pudlak syndrome type 7. This gene may also be associated with schizophrenia. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DTNBP1: The BLOC-1 complex is required for normal biogenesis of lysosome-related organelles, such as platelet dense granules and melanosomes. Plays a role in intracellular vesicle trafficking. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. May be required for normal dopamine homeostasis in the cerebral cortex, hippocampus, and hypothalamus. Plays a role in the regulation of cell surface exposure of DRD2. Contributes to the regulation of dopamine signaling. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Part of the biogenesis of lysosome-related organelles complex 1 (BLOC-1). The BLOC-1 complex is composed of BLOC1S1, BLOC1S2, BLOC1S3, DTNBP1, MUTED, PLDN, CNO/cappuccino and SNAPIN. Binds to DTNA and DTNB but may not be a physiological binding partner (PubMed:16980328). Interacts with RNF151 and CMYA5. Identified in a complex with the adapter-related protein complex 3 (AP-3). Interacts with TRIM32, AP3M1 and AP3B2. Identified in a complex with the biogenesis of lysosome-related organelles complex 2 (BLOC-2). Interacts with the DNA-dependent protein kinase complex DNA-PK. Detected in brain, in neurons and in neuropil. Detected in dentate gyrus and in pyramidal cells of hippocampus CA2 and CA3. Belongs to the dysbindin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: endoplasmic reticulum membrane; neuron projection; synaptic vesicle membrane; melanosome membrane; postsynaptic density; dendritic spine; cytosol; postsynaptic membrane; growth cone; axon; cytoplasm; endosome membrane; nucleus; sarcoplasm; cell junction; sarcolemma

Molecular Function: protein binding

Biological Process: platelet dense granule organization and biogenesis; melanosome organization and biogenesis; regulation of dopamine receptor signaling pathway; actin cytoskeleton reorganization; anterograde synaptic vesicle transport; post-Golgi vesicle-mediated transport; blood coagulation; anterograde axon cargo transport; positive regulation of neurotransmitter secretion; regulation of dopamine secretion; neurite development; neurite morphogenesis

Disease: Schizophrenia; Hermansky-pudlak Syndrome 7