Rabbit DRAM Polyclonal Antibody | anti-DRAM antibody

DRAM (HRP)

Cat. Num. AAA6444122

• Gene Names: DRAM1; DRAM
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Immunoaffinity chromatography.
• Synonyms: DRAM; Polyclonal Antibody; DRAM (HRP); anti-DRAM antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DRAM. Species Crossreactivity: Mouse, rat
• Purity/Purification: Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DRAM antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 2.5ug/ml; WB: 0.5-1.0ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to 16aa of human DRAM at C-terminal.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.

Western Blot (WB)

(Western blot analysis of whole cell lysate from HEK293 human epithelial kidney cells probed with Rabbit anti Human DRAM at 0.5(A) and 1(B)ug/ml)

Immunohistochemistry (IHC)

(Immunohistochemical staining of human liver with Rabbit anti Human DRAM)

Product Categories/Family for anti-DRAM antibody

Antibodies; Autophagy

References

1. Crighton D, et al. (2007). DRAM links autophagy to p53 and programmed cell death. Autophagy 3:72-4 2. Crighton D, et al (2006). DRAM, a p53-induced modulator of autophagy, is critical for apoptosis. Cell 126:121-34. 3. Gozuacik D., et al (2004). Autophagy as a cell death and tumor suppressor mechanism. Oncogene 23:2891-906.

NCBI and Uniprot Product Information

• NCBI GI #: 22450862
• NCBI GeneID: 55332
• UniProt Accession #: Q8N682; Q7L3E3; Q9NUN1; B7Z4T0
• Molecular Weight: 13,972 Da
• NCBI Official Full Name: Damage-regulated autophagy modulator
• NCBI Official Synonym Full Names: DNA-damage regulated autophagy modulator 1
• NCBI Official Symbol: DRAM1
• NCBI Official Synonym Symbols: DRAM
• NCBI Protein Information: DNA damage-regulated autophagy modulator protein 1
• UniProt Protein Name: DNA damage-regulated autophagy modulator protein 1
• UniProt Gene Name: DRAM1
• UniProt Synonym Gene Names: DRAM
• UniProt Entry Name: DRAM1_HUMAN

NCBI Description

This gene is regulated as part of the p53 tumor suppressor pathway. The gene encodes a lysosomal membrane protein that is required for the induction of autophagy by the pathway. Decreased transcriptional expression of this gene is associated with various tumors. This gene has a pseudogene on chromosome 4. [provided by RefSeq, Jul 2008]

Uniprot Description

DRAM1: Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73- mediated autophagy. Belongs to the DRAM/TMEM150 family.

Protein type: Apoptosis; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q23.2

Cellular Component: lysosome; lysosomal membrane; cytoplasm; integral to membrane

Biological Process: apoptosis; autophagy; regulation of autophagy