Western Blot (WB)
(DPPA5 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DPPA5 antibody detected the DPPA5 protein (arrow).)
Rabbit anti-Human DPPA5 Polyclonal Antibody | anti-DPPA5 antibody
DPPA5, NT (DPPA5, ESG1, Developmental pluripotency-associated 5 protein, Embryonal stem cell-specific gene 1 protein)
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Western Blot (WB)
(DPPA5 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DPPA5 antibody detected the DPPA5 protein (arrow).)
Western Blot (WB)
(DPPA5 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the DPPA5 antibody detected the DPPA5 protein (arrow).)
Flow Cytometry (FC/FACS)
(DPPA5 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(DPPA5 Antibody (N-term) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that may function in the control of cell pluripotency and early embryogenesis. Expression of this gene is a specific marker for pluripotent stem cells. Pseudogenes of this gene are located on the short arm of chromosome 10 and the long arm of chromosomes 14 and 19. [provided by RefSeq, Dec 2010]
Uniprot Description
Function: Involved in the maintenance of embryonic stem (ES) cell pluripotency. Dispensable for self-renewal of pluripotent ES cells and establishment of germ cells. Associates with specific target mRNAs
By similarity.
Subcellular location: Cytoplasm
By similarity.
Developmental stage: Expressed in primordial germ (PGC), embryonic germ (EGC) and embryonic stem (ESC) cells. Not detected in embryonic carcinoma (ECC) cells. Ref.4
Induction: Down-regulated in differentiated embryonic stem (ESC) cells, compared with strong expression in undifferentiated ESC cells. Ref.4
Sequence similarities: Belongs to the KHDC1 family.Contains 1 KH domain.
Research Articles on DPPA5
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Product Notes
The DPPA5 dppa5 (Catalog #AAA645571) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DPPA5, NT (DPPA5, ESG1, Developmental pluripotency-associated 5 protein, Embryonal stem cell-specific gene 1 protein) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DPPA5 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the DPPA5 dppa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DPPA5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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