Rabbit anti-Human DPH2 Polyclonal Antibody | anti-DPH2 antibody

DPH2 (Diphthamide Biosynthesis Protein 2, DPH2 Homolog, HsDph2, Diphthamide Biosynthesis Protein 2 Homolog-like 2, DPH2-like 2, DPH2L2) (AP)

Cat. Num. AAA6376535

• Gene Names: DPH2; DPH2L2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DPH2; Polyclonal Antibody; DPH2 (Diphthamide Biosynthesis Protein 2; DPH2 Homolog; HsDph2; Diphthamide Biosynthesis Protein 2 Homolog-like 2; DPH2-like 2; DPH2L2) (AP); anti-DPH2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DPH2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
• Sequence Length: 489

• Applicable Applications for anti-DPH2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human DPH2, aa1-489 (NP_001375.2).
• Immunogen Sequence: MESMFSSPAEAALQRETGVPGLLTPLPDLDGVYELERVAGFVRDLGCERVALQFPDQLLGDAVAVAARLEETTGSKMFILGDTAYGSCCVDVLGAEQAGAQALIHFGPACLSPPARPLPVAFVLRQRSVALELCVKAFEAQNPDPKAPVVLLSEPACAHALEALATLLRPRYLDLLVSSPAFPQPVGSLSPEPMPLERFGRRFPLAPGRRLEEYGAFYVGGSKASPDPDLDPDLSRLLLGWAPGQPFSSCCPDTGKTQDEGARAGRLRARRRYLVERARDARVVGLLAGTLGVAQHREALAHLRNLTQAAGKRSYVLALGRPTPAKLANFPEVDVFVLLACPLGALAPQLSGSFFQPILAPCELEAACNPAWPPPGLAPHLTHYADLLPGSPFHVALPPPESELWETPDVSLITGDLRPPPAWKSSNDHGSLALTPRPQLELAESSPAASFLSSRSWQGLEPRLGQTPVTEAVSGRRGIAIAYEDEGSG
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of DPH2 expression in transfected 293T cell line by DPH2 polyclonal antibody. Lane 1: DPH2 transfected lysate (52.1kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-DPH2 antibody

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2.

Product Categories/Family for anti-DPH2 antibody

Antibodies; Oncoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 21536476
• NCBI GeneID: 1802
• NCBI Accession #: NP_001375.2
• NCBI GenBank Nucleotide #: NM_001384.4
• UniProt Accession #: Q9BQC3
• NCBI Official Full Name: 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2 isoform a
• NCBI Official Synonym Full Names: diphthamide biosynthesis 2
• NCBI Official Symbol: DPH2
• NCBI Official Synonym Symbols: DPH2L2
• NCBI Protein Information: 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2
• UniProt Protein Name: 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2
• Protein Family: 2-(3-amino-3-carboxypropyl)histidine synthase
• UniProt Gene Name: DPH2
• UniProt Synonym Gene Names: DPH2L2

NCBI Description

This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]

Uniprot Description

Required for the first step in the synthesis of diphthamide, a post-translational modification of histidine which occurs in translation elongation factor 2 (EEF2).