Rabbit anti-Human DPF3 Polyclonal Antibody | anti-DPF3 antibody

DPF3, ID (DPF3, BAF45C, CERD4, Zinc finger protein DPF3, BRG1-associated factor 45C, Zinc finger protein cer-d4) (APC)

Cat. Num. AAA6293313

• Gene Names: DPF3; CERD4; BAF45C
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DPF3; Polyclonal Antibody; ID (DPF3; BAF45C; CERD4; Zinc finger protein DPF3; BRG1-associated factor 45C; Zinc finger protein cer-d4) (APC); Zinc finger protein cer-d4; anti-DPF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DPF3 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DPF3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 150-180 amino acids from the Central region of human DPF3.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DPF3 Antibody (Center) western blot analysis in ZR-75-1 cell line lysates (35ug/lane).This demonstrates the DPF3 antibody detected the DPF3 protein (arrow).)

Product Categories/Family for anti-DPF3 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

References

Lange, M., et al. Genes Dev. 22(17):2370-2384(2008). Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008). Hoyal, C.R., et al. J Carcinog 4, 13 (2005): Ninkina, N.N., et al. Mamm. Genome 12(11):862-866(2001). Harrington, J.J., et al. Nat. Biotechnol. 19(5):440-445(2001). Chestkov, A.V., et al. Genomics 36(1):174-177(1996).

NCBI and Uniprot Product Information

• NCBI GI #: 148762958
• NCBI GeneID: 8110
• NCBI Accession #: NP_036206.3
• NCBI GenBank Nucleotide #: NM_012074.4
• UniProt Accession #: Q92784; Q32UJ0; Q6P9E6; Q6ZT41; Q9H7Y5; A8MSI3; B7Z276; F5H575
• Molecular Weight: 41,184 Da
• NCBI Official Full Name: zinc finger protein DPF3 isoform 1
• NCBI Official Synonym Full Names: double PHD fingers 3
• NCBI Official Symbol: DPF3
• NCBI Official Synonym Symbols: CERD4; BAF45C
• NCBI Protein Information: zinc finger protein DPF3
• UniProt Protein Name: Zinc finger protein DPF3
• Protein Family: Zinc finger protein
• UniProt Gene Name: DPF3
• UniProt Synonym Gene Names: BAF45C; CERD4; BAF45C
• UniProt Entry Name: DPF3_HUMAN

NCBI Description

This gene encodes a member of the D4 protein family. The encoded protein is a transcription regulator that binds acetylated histones and is a component of the BAF chromatin remodeling complex. Alternate splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

DPF3: Belongs to the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. Muscle-specific component of the BAF complex, a multiprotein complex involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Specifically binds acetylated lysines on histone 3 and 4 (H3K14ac, H3K9ac, H4K5ac, H4K8ac, H4K12ac, H4K16ac). In the complex, it acts as a tissue-specific anchor between histone acetylations and methylations and chromatin remodeling. It thereby probably plays an essential role in heart and skeletal muscle development. Belongs to the requiem/DPF family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 14q24.2

Molecular Function: zinc ion binding

Biological Process: nervous system development; transcription, DNA-dependent; regulation of transcription, DNA-dependent; chromatin modification