Rabbit DPF2 Polyclonal Antibody | anti-DPF2 antibody

DPF2 (E174) Polyclonal Antibody

Cat. Num. AAA3002914

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DPF2; Polyclonal Antibody; DPF2 (E174) Polyclonal Antibody; Apoptosis response zinc finger protein; BAF45D; BRG1-associated factor 45D; D4; D4 zinc and double PHD fingers family 2; double PHD fingers family 2; DPF 2; MGC10180; Protein requiem; REQ; REQU; Requiem; Requiem apoptosis response zinc finger; UBI D4; ubi-d4; UBID 4; UBID4; zinc and double PHD fingers family 2; Zinc finger protein ubi d4; anti-DPF2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: DPF2 (E174) polyclonal antibody detects endogenous levels of DPF2 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 391

• Applicable Applications for anti-DPF2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 140-188 of Human DPF2.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DPF2 (E174) polyclonal antibody at 1:1000 dilution Lane1:THP-1 whole cell lysate (37ug) Lane2:MCF-7 whole cell lysate (48ug) Lane3:RAW264. 7 whole cell lysate (57ug) Lane4:NIH3T3 whole cell lysate (48ug) Lane5:H9C2 whole cell lysate (37ug))

Western Blot (WB)

(Western Blot (WB) analysis of DPF2 (E174) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (20ug) Lane2:RAW264. 7 whole cell lysate (20ug) Lane3:H9C2 whole cell lysate (20ug))

Related Product Information for anti-DPF2 antibody

DPF2 (D4, zinc and double PHD fingers family 2), also known as REQ (Requiem), UBID4 or ubi-d4, is a 391 amino acid protein that is a member of the D4 domain family. DPF2, a ubiquitously expressed protein, localizes to the nucleus and contains one C2H2- and two PHD-type zinc finger motifs. DPF2 may function as a transcription factor that is necessary for apoptosis and may also play a role in the development and maturation of lymphoid cells. It is thought that, during apoptosis, DPF2 activity is inhibited by LRF (Leukemia/lymphoma-related factor), which is upregulated by integrin. This suggests that DPF2 may be a potential target for future cancer therapies that induce apoptosis in leukemia cells. Alternative splicing of this gene generates multiple isoforms lacking certain domain.

NCBI and Uniprot Product Information

• NCBI GI #: 5454004
• NCBI Accession #: NP_006259.1
• NCBI GenBank Nucleotide #: NP_006259.1
• UniProt Accession #: Q92785
• Molecular Weight: ~ 44kDa
• NCBI Official Full Name: zinc finger protein ubi-d4 isoform 1
• UniProt Protein Name: Zinc finger protein ubi-d4
• Protein Family: Zinc finger protein
• UniProt Gene Name: DPF2
• UniProt Synonym Gene Names: BAF45D; REQ; UBID4; BAF45D
• UniProt Entry Name: REQU_HUMAN

Uniprot Description

requiem: May be a transcription factor required for the apoptosis response following survival factor withdrawal from myeloid cells. Might also have a role in the development and maturation of lymphoid cells. Belongs to the requiem/DPF family.

Protein type: Apoptosis; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: nucleoplasm; centrosome; intracellular membrane-bound organelle; cytoplasm; nuclear chromatin

Molecular Function: zinc ion binding

Biological Process: regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis