Western Blot (WB)
(DP1 antibody (pAb) tested by Western blot. Raji Nuclear cell extract (20 ug) probed with DP1 antibody at a dilution of 1:1,000.)
Rabbit anti-Human DP1 Polyclonal Antibody | anti-DP1 antibody
DP1 antibody (pAb)
WB: 1:500-1:2,000 dilution
Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot.
Western Blot (WB)
(DP1 antibody (pAb) tested by Western blot. Raji Nuclear cell extract (20 ug) probed with DP1 antibody at a dilution of 1:1,000.)
Western Blot (WB)
(DP1 antibody (pAb) tested by Western blot. Raji Nuclear cell extract (20 ug) probed with DP1 antibody at a dilution of 1:1,000.)
Background: DP1 (DRTF1 polypeptide 1, TFDP1) is a transcriptional co-activator and an important regulator of cell cycle progression. It is a member of a protein family first identified through their binding to E2F DNA binding sites. DP1 forms heterodimers with E2F family members, activating transcription of E2F-responsive genes. DP1 is also absolutely required for extraembryonic development and embryonic survival.
NCBI and Uniprot Product Information
Uniprot Description
TFDP1: Can stimulate E2F-dependent transcription. Binds DNA cooperatively with E2F family members through the E2 recognition site, 5'-TTTC[CG]CGC-3', found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DP2/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F1/DP complex appears to mediate both cell proliferation and apoptosis. Belongs to the E2F/DP family.
Protein type: DNA-binding; Transcription, coactivator/corepressor
Chromosomal Location of Human Ortholog: 13q34
Cellular Component: nucleoplasm; transcription factor complex
Molecular Function: protein domain specific binding; protein binding; DNA binding; transcription coactivator activity; transcription factor activity; transcription factor binding
Biological Process: transcription initiation from RNA polymerase II promoter; Notch signaling pathway; epidermis development; transcription, DNA-dependent; apoptosis; anoikis; regulation of transcription from RNA polymerase II promoter; cell proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; gene expression; mitotic cell cycle; G1/S transition of mitotic cell cycle
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Product Notes
The DP1 tfdp1 (Catalog #AAA388781) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DP1 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications Validated: WB: 1:500-1:2,000 dilution Note: Many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt/Sonication Protocol when preparing nuclear extracts for Western blot. Researchers should empirically determine the suitability of the DP1 tfdp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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