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Western Blot (WB) (Western blot analysis of Dopey-2 on mouse heart (1) and human heart (2) tissue lysate using anti-Dopey-2 antibody at 1/500 dilution.)

Rabbit anti-Human, Mouse DOPEY2 Polyclonal Antibody | anti-DOPEY2 antibody

DOPEY2 Antibody

Gene Names
DOPEY2; 21orf5; C21orf5
Reactivity
Human, Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Immunogen affinity purified
Synonyms
DOPEY2; Polyclonal Antibody; DOPEY2 Antibody; Dipeptidase 2 antibody; Dipeptidase2 antibody; DPEP 2 antibody; MBD 2 antibody; MBD2 antibody; anti-DOPEY2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Immunogen affinity purified
Form/Format
1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-DOPEY2 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:500-1:1000
IHC: 1:50-1:200
ICC: 1:50-1:200
FC/FACS: 1:50-1:100
Immunogen
Peptide
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of Dopey-2 on mouse heart (1) and human heart (2) tissue lysate using anti-Dopey-2 antibody at 1/500 dilution.)

Western Blot (WB) (Western blot analysis of Dopey-2 on mouse heart (1) and human heart (2) tissue lysate using anti-Dopey-2 antibody at 1/500 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue labeling Dopey-2. Counterstained with Hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse heart tissue labeling Dopey-2. Counterstained with Hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling Dopey-2. Counterstained with Hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling Dopey-2. Counterstained with Hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Dopey-2 in D3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining Dopey-2 in D3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of N2A cells with Dopey-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of N2A cells with Dopey-2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
Related Product Information for anti-DOPEY2 antibody
Dopey-2, a 2, 298 amino acid protein, belongs to the dopey family, that is overexpressed in lymphoblasts from Down syndrome patients and Abundantly expressed in developing central nervous system, with highest levels in cerebellum and lowest in telencephalon. Multiple isoforms of Dopey-2 exist due to alternative splicing events. Dopey-2 is thought to play a role in protein trafficking between early endosomes and the late.
Product Categories/Family for anti-DOPEY2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
260kDa
NCBI Official Full Name
protein dopey-2
NCBI Official Synonym Full Names
dopey family member 2
NCBI Official Symbol
DOPEY2
NCBI Official Synonym Symbols
21orf5; C21orf5
NCBI Protein Information
protein dopey-2
UniProt Protein Name
Protein dopey-2
Protein Family
UniProt Gene Name
DOPEY2
UniProt Synonym Gene Names
C21orf5; KIAA0933

Uniprot Description

May be involved in protein traffic between late Golgi and early endosomes.

Research Articles on DOPEY2

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Product Notes

The DOPEY2 dopey2 (Catalog #AAA9434902) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DOPEY2 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's DOPEY2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB: 1:500-1:1000 IHC: 1:50-1:200 ICC: 1:50-1:200 FC/FACS: 1:50-1:100. Researchers should empirically determine the suitability of the DOPEY2 dopey2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DOPEY2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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