Rabbit anti-Rat Dopamine Receptor, D1a, D1R Polyclonal Antibody | anti-DRD1 antibody

Dopamine Receptor, D1a, D1R

Cat. Num. AAA616198

• Gene Names: DRD1; DADR; DRD1A
• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Serum; Serum
• Synonyms: Dopamine Receptor; D1a; D1R; Polyclonal Antibody; Anti -Dopamine Receptor; anti-DRD1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the rat dopamine receptor D1a. No significant sequence homology with other dopamine receptors (D1B, D2-D5). Species Sequence Homology
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-DRD1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:500-1:2500 using ECL. Detects 2 proteins of 50 and 75kD in solubilized hippocampal membranes. The 75kD band was not visible in striatum (2). ~100-400ug protein/lane should be loaded to visualize the 75kD band.; ELISA: 1:5000-1:25,000. Coat ELISA plates at 1ug/ml of Control Peptide.
• Immunogen: Synthetic peptide 9aa sequence of rat D1R receptor within the C-terminus of the cytoplasmic domain (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-DRD1 antibody

Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. DR have been classified into either the D1-like (D1, D1B, and D5) or D2-like (D2, D3, and D4). The two isoforms of D2R, D2 long (D2L) and short D2S), are encoded by splice variants of a single gene and differ only by the presence of an additional 29aa in the intracellular domain 3 of the D2 long form. It may play a role in the coupling of the receptor to G-proteins. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell or tissue may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize, and document changes in DR levels in cells and tissues under various normal and pathological conditions.

Product Categories/Family for anti-DRD1 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4503383
• NCBI GeneID: 1812
• NCBI Accession #: NP_000785.1
• NCBI GenBank Nucleotide #: NM_000794.3
• UniProt Accession #: P21728; Q4QRJ0; B2RA44; Q6FH34
• Molecular Weight: 49,293 Da
• NCBI Official Full Name: D(1A) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D1
• NCBI Official Symbol: DRD1
• NCBI Official Synonym Symbols: DADR; DRD1A
• NCBI Protein Information: D(1A) dopamine receptor; D(1A) dopamine receptor; dopamine D1 receptor
• UniProt Protein Name: D(1A) dopamine receptor
• Protein Family: D(1A) dopamine receptor
• UniProt Gene Name: DRD1
• UniProt Entry Name: DRD1_HUMAN

NCBI Description

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq]

Uniprot Description

DRD1: a G-protein coupled receptor.One of the five types (D1 to D5) of receptors for dopamine. The most abundant dopamine receptor in the central nervous system. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Interacts with calcyon.

Protein type: GPCR, family 1; Receptor, GPCR; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; nucleus

Molecular Function: protein binding; dopamine receptor activity; dopamine binding; dopamine D1 receptor-like receptor activity

Biological Process: synaptic transmission, dopaminergic; peristalsis; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); behavioral fear response; prepulse inhibition; positive regulation of potassium ion transport; generation of action potential; thermoregulation; vasodilation; G-protein signaling, adenylate cyclase activating pathway; positive regulation of synaptic transmission, glutamatergic; adult walking behavior; dopamine metabolic process; synaptogenesis; protein import into nucleus; behavioral response to cocaine; conditioned taste aversion; positive regulation of cAMP biosynthetic process; visual learning; dopamine receptor, adenylate cyclase activating pathway; grooming behavior; response to drug; dopamine transport; dentate gyrus development; cerebral cortex GABAergic interneuron migration; striatum development; maternal behavior; regulation of dopamine metabolic process; response to amphetamine; adenylate cyclase activation; mating behavior; regulation of dopamine uptake; transmission of nerve impulse; astrocyte development; memory; G-protein signaling, coupled to cyclic nucleotide second messenger; habituation; glucose import; sensitization; operant conditioning; dopamine receptor, phospholipase C activating pathway; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cell migration