Rabbit anti-Rat Dopamine Receptor, D1a, aa403-415 Polyclonal Antibody | anti-DRD1 antibody

Dopamine Receptor, D1a, aa403-415

Cat. Num. AAA627205

• Gene Names: DRD1; DADR; DRD1A
• Reactivity: Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: Dopamine Receptor; D1a; aa403-415; Polyclonal Antibody; Anti -Dopamine Receptor; anti-DRD1 antibody

• Host: Rabbit
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes 2 rat proteins of 50 and 75kD in solubilized hippocampal membranes by Western blot. The 75kD band was not visible in striatum. Approximately 100-400 ug of protein/lane should be loaded to visualize the 75kD band. In IHC, D1R has been localized in various regions of the rat brain.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in 100mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide.

• Applicable Applications for anti-DRD1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western blot, Immunohistochemistry, and ELISA.; Dilution: Western Blot: 1-10ug/ml (Chemiluminescence technique) Immunohistochemistry: 2-10ug/ml on paraformaldehyde fixed sections.; ELISA: 0.5-1.0ug/ml (1ug/ml D1A peptide/well); Optimal dilution determined by the researcher.
• Immunogen: A 13 amino acid peptide sequence (amino acids 403-415) from rat D1a (1). This peptide is predicted to be cytoplasmic.
• Species Sequence Homology: Porcine or bovine (100%), human (78%) and mouse (92%).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-DRD1 antibody

The rat D1a receptor is a 446 amino acid, G-protein coupled and transmembrane receptor protein

Product Categories/Family for anti-DRD1 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4503383
• NCBI GeneID: 1812
• NCBI Accession #: NP_000785.1
• NCBI GenBank Nucleotide #: NM_000794.3
• UniProt Accession #: P21728; Q4QRJ0; B2RA44
• Molecular Weight: 49,293 Da
• NCBI Official Full Name: D(1A) dopamine receptor
• NCBI Official Synonym Full Names: dopamine receptor D1
• NCBI Official Symbol: DRD1
• NCBI Official Synonym Symbols: DADR; DRD1A
• NCBI Protein Information: D(1A) dopamine receptor; D(1A) dopamine receptor; dopamine D1 receptor
• UniProt Protein Name: D(1A) dopamine receptor
• Protein Family: D(1A) dopamine receptor
• UniProt Gene Name: DRD1
• UniProt Entry Name: DRD1_HUMAN

NCBI Description

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

DRD1: a G-protein coupled receptor.One of the five types (D1 to D5) of receptors for dopamine. The most abundant dopamine receptor in the central nervous system. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Interacts with calcyon.

Protein type: Membrane protein, multi-pass; Receptor, GPCR; Membrane protein, integral; GPCR, family 1

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; nucleus

Molecular Function: protein binding; dopamine receptor activity; dopamine binding; dopamine D1 receptor-like receptor activity

Biological Process: synaptic transmission, dopaminergic; peristalsis; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); behavioral fear response; prepulse inhibition; positive regulation of potassium ion transport; generation of action potential; thermoregulation; adult walking behavior; vasodilation; G-protein signaling, adenylate cyclase activating pathway; dopamine metabolic process; positive regulation of synaptic transmission, glutamatergic; synaptogenesis; behavioral response to cocaine; protein import into nucleus; conditioned taste aversion; positive regulation of cAMP biosynthetic process; visual learning; dopamine receptor, adenylate cyclase activating pathway; response to drug; grooming behavior; dopamine transport; dentate gyrus development; striatum development; cerebral cortex GABAergic interneuron migration; maternal behavior; response to amphetamine; adenylate cyclase activation; regulation of dopamine metabolic process; regulation of dopamine uptake; mating behavior; transmission of nerve impulse; astrocyte development; memory; G-protein signaling, coupled to cyclic nucleotide second messenger; habituation; glucose import; sensitization; operant conditioning; positive regulation of release of sequestered calcium ion into cytosol; dopamine receptor, phospholipase C activating pathway; positive regulation of cell migration