Rabbit Dopamine Receptor, D1, aa9-21 Polyclonal Antibody | anti-DRD1 antibody

Dopamine Receptor, D1, aa9-21

Cat. Num. AAA625302

• Gene Names: DRD1; DADR; DRD1A
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Serum; Serum
• Synonyms: Dopamine Receptor; D1; aa9-21; Polyclonal Antibody; Anti -Dopamine Receptor; anti-DRD1 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes D1 Dopamine Receptor (9-21) 100% Crossreactivity: D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10); 0% D3 Dopamine Receptor; 0% D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; D5 Dopamine Receptor 0%
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a lyophillized powder. Reconstitute with 100ul sterile PBS, 10mg/ml BSA or with additional buffer for more dilute antisera.

• Applicable Applications for anti-DRD1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunohistochemistry, Western Blot, and ELISA.; Dilution: Western Blot: 1:1000 (whole rat brain homogenate resulted in a single band being detected at 50kD); ELISA: 1:5000.; Immunohistochemistry: 1:5000. (staining cells in various regions of PLP fixed rat brain sections, inlcuding Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area.)
• Immunogen: D1 (Ac-9-21-Cys22) covalently attached to a carrier protein.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 10mg/ml BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-DRD1 antibody

Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport. A family of at least 5 Dopamine Receptors (DR) genes, D1-D5, have been identified based upon the amino acid identity, pharmacological specificity and effector responses. All members of this family have similar structure and contain 7 putative transmembrane domains. A given cell or tissue may express more than one DR. Specific radioligands do not exist that can differentiate between these DR. Therefore, specific antibodies are needed to distinguish, localize and document changes in DR levels in cells and tissues under various normal and pathological conditions.

Product Categories/Family for anti-DRD1 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 20269979
• NCBI GeneID: 1812
• UniProt Accession #: P21728; Q4QRJ0; B2RA44; Q6FH34
• Molecular Weight: 49,293 Da
• NCBI Official Full Name: dopamine receptor D1
• NCBI Official Synonym Full Names: dopamine receptor D1
• NCBI Official Symbol: DRD1
• NCBI Official Synonym Symbols: DADR; DRD1A
• NCBI Protein Information: D(1A) dopamine receptor; D(1A) dopamine receptor; dopamine D1 receptor
• UniProt Protein Name: D(1A) dopamine receptor
• Protein Family: D(1A) dopamine receptor
• UniProt Gene Name: DRD1
• UniProt Entry Name: DRD1_HUMAN

NCBI Description

This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. [provided by RefSeq]

Uniprot Description

DRD1: a G-protein coupled receptor.One of the five types (D1 to D5) of receptors for dopamine. The most abundant dopamine receptor in the central nervous system. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. Interacts with calcyon.

Protein type: Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 5q35.1

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; nucleus

Molecular Function: protein binding; dopamine receptor activity; dopamine binding; dopamine D1 receptor-like receptor activity

Biological Process: synaptic transmission, dopaminergic; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); peristalsis; behavioral fear response; prepulse inhibition; positive regulation of potassium ion transport; generation of action potential; thermoregulation; adult walking behavior; dopamine metabolic process; G-protein signaling, adenylate cyclase activating pathway; positive regulation of synaptic transmission, glutamatergic; vasodilation; synaptogenesis; behavioral response to cocaine; protein import into nucleus; conditioned taste aversion; positive regulation of cAMP biosynthetic process; visual learning; dopamine receptor, adenylate cyclase activating pathway; response to drug; grooming behavior; dopamine transport; dentate gyrus development; striatum development; cerebral cortex GABAergic interneuron migration; maternal behavior; response to amphetamine; adenylate cyclase activation; regulation of dopamine metabolic process; regulation of dopamine uptake; mating behavior; transmission of nerve impulse; astrocyte development; memory; G-protein signaling, coupled to cyclic nucleotide second messenger; habituation; glucose import; sensitization; operant conditioning; dopamine receptor, phospholipase C activating pathway; positive regulation of release of sequestered calcium ion into cytosol; positive regulation of cell migration