Western Blot (WB)
((0. 03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat DOPA decarboxylase Polyclonal Antibody | anti-DDC antibody
Goat anti-DOPA decarboxylase Antibody
Western Blot: Approx 50kDa band observed in Human Kidney lysates and 48kDa on Human Brain lysates (calculated MW of 53.9kDa according to NP_000781.1). Recommended concentration: 0. 03-0.1ug/ml.
Western Blot (WB)
((0. 03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0. 03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI Description
The encoded protein catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Defects in this gene are the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD). AADCD deficiency is an inborn error in neurotransmitter metabolism that leads to combined serotonin and catecholamine deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2011]
Uniprot Description
DDC: Catalyzes the decarboxylation of L-3,4- dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine. Homodimer. Belongs to the group II decarboxylase family.
Protein type: Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - phenylalanine; Nuclear receptor co-regulator; EC 4.1.1.28; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - histidine; Lyase
Chromosomal Location of Human Ortholog: 7p12.2
Cellular Component: axon; cell soma; cytosol; synaptic vesicle
Molecular Function: amino acid binding; aromatic-L-amino-acid decarboxylase activity; enzyme binding; protein binding; protein domain specific binding; pyridoxal phosphate binding
Biological Process: amino acid metabolic process; catecholamine biosynthetic process; circadian rhythm; dopamine biosynthetic process; indolalkylamine biosynthetic process; isoquinoline alkaloid metabolic process; multicellular organismal aging; phytoalexin metabolic process; response to pyrethroid; serotonin biosynthetic process; synaptic vesicle amine transport
Disease: Aromatic L-amino Acid Decarboxylase Deficiency
Research Articles on DDC
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Product Notes
The DDC ddc (Catalog #AAA420200) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-DOPA decarboxylase Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's DOPA decarboxylase can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 50kDa band observed in Human Kidney lysates and 48kDa on Human Brain lysates (calculated MW of 53.9kDa according to NP_000781.1). Recommended concentration: 0. 03-0.1ug/ml. Researchers should empirically determine the suitability of the DDC ddc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: WEHIKELAAD VL. It is sometimes possible for the material contained within the vial of "DOPA decarboxylase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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