Rabbit DOK4 Polyclonal Antibody | anti-DOK4 antibody

DOK4 (Docking Protein 4, Downstream of Kinase-4, FLJ10488, Insulin Receptor Substrate 5, IRS5, IRS 5) (PE)

Cat. Num. AAA6414615

• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: DOK4; Polyclonal Antibody; DOK4 (Docking Protein 4; Downstream of Kinase-4; FLJ10488; Insulin Receptor Substrate 5; IRS5; IRS 5) (PE); anti-DOK4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes DOK4. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-DOK4 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:500-1:2000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Recombinant protein corresponding to human DOK4.
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of MCF7 cells, using DOK4 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded Prostate cancer using DOK4 Antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human stomach cancer using DOK4 antibody at dilution of 1:200 (200x lens).)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded mouse kidney using DOK4 antibody at dilution of 1:200 (200x lens).)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using DOK4 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-DOK4 antibody

Antibodies; Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 20070302
• NCBI GeneID: 55715
• NCBI Accession #: NP_060580.2
• NCBI GenBank Nucleotide #: NM_018110.3
• UniProt Accession #: Q8TEW6; O75209; Q9BTP2; Q9NVV3
• Molecular Weight: 37,028 Da
• NCBI Official Full Name: docking protein 4
• NCBI Official Synonym Full Names: docking protein 4
• NCBI Official Symbol: DOK4
• NCBI Protein Information: docking protein 4; IRS5; IRS-5; insulin receptor substrate 5; downstream of tyrosine kinase 4
• UniProt Protein Name: Docking protein 4
• Protein Family: Docking protein
• UniProt Gene Name: DOK4
• UniProt Synonym Gene Names: IRS-5; IRS5
• UniProt Entry Name: DOK4_HUMAN

Uniprot Description

DOK4: docking proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK4 functions in Ret-mediated neurite outgrowth and plays a positive role in activation of the MAP kinase pathway. Putative link with downstream effectors of Ret in neuronal differentiation. May be involved in the regulation of the immune response induced by T-cells. Interacts with Ret and TIE2. Interaction with Ret is mediated through the PTB domain and requires phosphorylation of Ret Y1062.

Protein type: Adaptor/scaffold; Cell development/differentiation

Chromosomal Location of Human Ortholog: 16q21

Molecular Function: protein binding; receptor signaling protein activity; insulin receptor binding

Biological Process: nervous system development; MAPKKK cascade; transmembrane receptor protein tyrosine kinase signaling pathway