Rabbit anti-Human Dnmt3b Polyclonal Antibody | anti-Dnmt3b antibody

Dnmt3b (DNMT3B, DNA (cytosine-5)-methyltransferase 3B, DNA methyltransferase HsaIIIB) (FITC)

Cat. Num. AAA6293117

• Gene Names: DNMT3B; ICF; ICF1; M.HsaIIIB
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography.
• Synonyms: Dnmt3b; Polyclonal Antibody; Dnmt3b (DNMT3B; DNA (cytosine-5)-methyltransferase 3B; DNA methyltransferase HsaIIIB) (FITC); DNMT3B; DNA methyltransferase HsaIIIB; anti-Dnmt3b antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Dnmt3b.
• Purity/Purification: Purified by Protein A/G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Dnmt3b antibody: Immunohistochemistry (IHC) Paraffin, FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 389-417 of human Dnmt3b, UniProt Accession #Q9UBC3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in T47D whole cell lysates (20ug/lane) using 034756 (1:2000). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 95kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(All lanes: 034756 (1:2000) Lane 1: A431 whole cell lysates Lane 2: A549 whole cell lysates Lysates/proteins at 20ug/lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10,000. Predicted band size: 95kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with 034756, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of 034756 for immunohistochemistry.)

Product Categories/Family for anti-Dnmt3b antibody

Antibodies; Enzymes; Methyltransferase

References

1. Okano, M., et al., Cell 99(3):247-257 (1999). 2. Yin, B., et al., Zhongguo Yi Xue Ke Xue Yuan Xue Bao 21(6):431-438 (1999). 3. Okano, M., et al., Nat. Genet. 19(3):219-220 (1998).

NCBI and Uniprot Product Information

• NCBI GI #: 333440487
• NCBI GeneID: 1789
• NCBI Accession #: NP_001193984.1
• NCBI GenBank Nucleotide #: NM_001207055.1
• UniProt Accession #: Q9UBC3; Q9UBD4; Q9UJQ5; Q9UKA6; Q9UNE5; A2A2E2; B4DSM8; B4DSU1; E1P5M6; E1P5M7; E7EN63; E9PBF2
• Molecular Weight: 95,751 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3B isoform 7
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 3 beta
• NCBI Official Symbol: DNMT3B
• NCBI Official Synonym Symbols: ICF; ICF1; M.HsaIIIB
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3B; DNA (cytosine-5)-methyltransferase 3B; DNA MTase HsaIIIB; DNA methyltransferase HsaIIIB
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3B
• Protein Family: DNA
• UniProt Gene Name: DNMT3B
• UniProt Synonym Gene Names: Dnmt3b; DNA MTase HsaIIIB; M.HsaIIIB
• UniProt Entry Name: DNM3B_HUMAN

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq, May 2011]

Uniprot Description

DNMT3B: a ubiquitous DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Numerous cancer cell lines and primary acute leukemias express aberrant DNMT3B transcripts, especially DNMT3B7. Transfection of DNMT3B7 into 293 cells alters the pattern of genes expressed in the transfected cells. Can interact with DNMT1, which might be a co-operative event during DNA methylation. Methylates CpG sites at a rate slower than DNMT3A and much slower than DNMT1. Interacts with SUV39H1, SETDB1, SUMO1 and UBE2I9. Interacts with DNMT1 and DNMT3A. Mutations in DNMT3B have been shown to cause immunodeficiency-centromeric instability-facial anomalies syndrome (ICF). Six alternatively spliced isoforms of the human protein have been reported. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate. Isoforms 4 and 5 are probably not enzymatically active due to the deletion of two conserved methyltransferase motifs.

Protein type: Methyltransferase; Methyltransferase, DNA; Cell development/differentiation; EC 2.1.1.37; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear heterochromatin; nucleus

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; unmethylated CpG binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; metal ion binding; DNA-methyltransferase activity; transcription corepressor activity

Biological Process: response to drug; negative regulation of histone H3-K9 methylation; genetic imprinting; S-adenosylhomocysteine metabolic process; positive regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; cytosine methylation within a CG sequence; protein complex localization; methylation-dependent chromatin silencing; negative regulation of gene expression, epigenetic; DNA methylation; S-adenosylmethioninamine metabolic process; gene expression; response to ionizing radiation; positive regulation of neuron differentiation; DNA methylation on cytosine

Disease: Immunodeficiency-centromeric Instability-facial Anomalies Syndrome 1