Western Blot (WB)
(DNAJC27 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the DNAJC27 antibody detected the DNAJC27 protein (arrow).)
Rabbit anti-Human DNAJC27 Polyclonal Antibody | anti-DNAJC27 antibody
DNAJC27, NT (DNAJC27, RABJS, RBJ, DnaJ homolog subfamily C member 27, Rab and DnaJ domain-containing protein) (AP)
Gene Names
DNAJC27; RBJ; RabJS
Reactivity
Human
Applications
Immunohistochemistry, ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
DNAJC27; Polyclonal Antibody; NT (DNAJC27; RABJS; RBJ; DnaJ homolog subfamily C member 27; Rab and DnaJ domain-containing protein) (AP); Rab and DnaJ domain-containing protein; anti-DNAJC27 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-DNAJC27 antibody
Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
Application Notes
IHC: 1:10-50
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
ELISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunogen
DNAJC27 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DNAJC27.
Conjugate
AP
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(DNAJC27 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the DNAJC27 antibody detected the DNAJC27 protein (arrow).)
Western Blot (WB)
(DNAJC27 Antibody (N-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the DNAJC27 antibody detected the DNAJC27 protein (arrow).)
Immunohistochemistry (IHC)
(DNAJC27 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNAJC27 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(DNAJC27 Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DNAJC27 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Related Product Information for anti-DNAJC27 antibody
Belongs to the small GTPase superfamily. Rab family. 3 isoforms.
Product Categories/Family for anti-DNAJC27 antibody
References
Gudbjartsson, D.F., et al. Nat. Genet. 40(5):609-615(2008). Nepomuceno-Silva, J.L., et al. Gene 327(2):221-232(2004).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20,264 Da
NCBI Official Full Name
dnaJ homolog subfamily C member 27 isoform 1
NCBI Official Synonym Full Names
DnaJ (Hsp40) homolog, subfamily C, member 27
NCBI Official Symbol
DNAJC27
NCBI Official Synonym Symbols
RBJ; RabJS
NCBI Protein Information
dnaJ homolog subfamily C member 27; Ras-associated protein Rap1; rab and DnaJ domain-containing protein
UniProt Protein Name
DnaJ homolog subfamily C member 27
Protein Family
UniProt Gene Name
DNAJC27
UniProt Synonym Gene Names
RABJS; RBJ
Uniprot Description
GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.
Research Articles on DNAJC27
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Product Notes
The DNAJC27 dnajc27 (Catalog #AAA6292817) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DNAJC27, NT (DNAJC27, RABJS, RBJ, DnaJ homolog subfamily C member 27, Rab and DnaJ domain-containing protein) (AP) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNAJC27 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB). IHC: 1:10-50 ELISA: 1:1,000 WB: 1:100-500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the DNAJC27 dnajc27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNAJC27, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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