Rabbit anti-Human DNAI1 Polyclonal Antibody | anti-DNAI1 antibody

DNAI1, NT (DNAI1, Dynein intermediate chain 1, axonemal, Axonemal dynein intermediate chain 1) (Azide free) (HRP)

Cat. Num. AAA6292579

• Gene Names: DNAI1; PCD; DIC1; ICS1; CILD1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DNAI1; Polyclonal Antibody; NT (DNAI1; Dynein intermediate chain 1; axonemal; Axonemal dynein intermediate chain 1) (Azide free) (HRP); Axonemal dynein intermediate chain 1; anti-DNAI1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DNAI1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DNAI1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 91-120 amino acids from the N-terminal region of human DNAI1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DNAI1 Antibody (N-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DNAI1 antibody detected the DNAI1 protein (arrow).)

Related Product Information for anti-DNAI1 antibody

The inner-and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. This gene encodes an intermediate chain dynein, belonging to the large family of motor proteins. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia (PCD) and Kartagener syndrome.

Product Categories/Family for anti-DNAI1 antibody

Antibodies; Disease Markers

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Leigh, M.W., et al. Curr. Opin. Pediatr. 21(3):320-325(2009). Zuccarello, D., et al. Hum. Reprod. 23(8):1957-1962(2008). Failly, M., et al. Respiration 76(2):198-204(2008). Zariwala, M.A., et al. Am. J. Respir. Crit. Care Med. 174(8):858-866(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 6912338
• NCBI GeneID: 27019
• NCBI Accession #: NP_036276.1
• NCBI GenBank Nucleotide #: NM_012144.3
• UniProt Accession #: Q9UI46; Q5T8G7; Q8NHQ7; Q9UEZ8; B7Z7U1
• Molecular Weight: 19,284 Da
• NCBI Official Full Name: dynein intermediate chain 1, axonemal isoform 1
• NCBI Official Synonym Full Names: dynein, axonemal, intermediate chain 1
• NCBI Official Symbol: DNAI1
• NCBI Official Synonym Symbols: PCD; DIC1; ICS1; CILD1
• NCBI Protein Information: dynein intermediate chain 1, axonemal; immotile cilia syndrome 1; dynein, axonemal, intermediate polypeptide 1
• UniProt Protein Name: Dynein intermediate chain 1, axonemal
• Protein Family: Dynein
• UniProt Gene Name: DNAI1
• UniProt Entry Name: DNAI1_HUMAN

NCBI Description

This gene encodes a member of the dynein intermediate chain family. The encoded protein is part of the dynein complex in respiratory cilia. The inner- and outer-arm dyneins, which bridge between the doublet microtubules in axonemes, are the force-generating proteins responsible for the sliding movement in axonemes. The intermediate and light chains, thought to form the base of the dynein arm, help mediate attachment and may also participate in regulating dynein activity. Mutations in this gene result in abnormal ciliary ultrastructure and function associated with primary ciliary dyskinesia and Kartagener syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]