Rabbit anti-Human DNA2 Polyclonal Antibody | anti-DNA2 antibody

Anti-DNA2 Antibody

Cat. Num. AAA7607586

• Gene Names: DNA2; DNA2L; hDNA2
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: >=95% as determined by SDS-PAGE; Immunogen affinity purified
• Synonyms: DNA2; Polyclonal Antibody; Anti-DNA2 Antibody; DNA2L; KIAA0083; anti-DNA2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: >=95% as determined by SDS-PAGE; Immunogen affinity purified
• Form/Format: Liquid; PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Sequence Length: 1060

• Applicable Applications for anti-DNA2 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: IHC: 1:20-1:200
• Immunogen: DNA replication helicase 2 homolog
• Preparation and Storage: Store at -20 degree C for 24 months. Avoid repeated freeze/thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7607586 (DNA2 antibody) at dilution of 1:100)

Related Product Information for anti-DNA2 antibody

Key enzyme involved in DNA replication and DNA repair in nucleus and mitochondrion. Involved in Okazaki fragments processing by cleaving long flaps that escape FEN1: flaps that are longer than 27 nucleotides are coated by replication protein A complex(RPA), leading to recruit DNA2 which cleaves the flap until it is too short to bind RPA and becomes a substrate for FEN1. Also involved in 5'-end resection of DNA during double-strand break(DSB) repair: recruited by BLM and mediates the cleavage of 5'-ssDNA, while the 3'-ssDNA cleavage is prevented by the presence of RPA. Also involved in DNA replication checkpoint independently of Okazaki fragments processing. Possesses different enzymatic activities, such as single-stranded DNA(ssDNA)-dependent ATPase, 5'-3' helicase and endonuclease activities. While the ATPase and endonuclease activities are well-defined and play a key role in Okazaki fragments processing and DSB repair, the 5'-3' DNA helicase activity is subject to debate. According to various reports, the helicase activity is weak and its function remains largely unclear. Helicase activity may promote the motion of DNA2 on the flap, helping the nuclease function.

Product Categories/Family for anti-DNA2 antibody

Metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 320461728
• NCBI GeneID: 1763
• NCBI Accession #: NP_001073918.2
• NCBI GenBank Nucleotide #: NP_001073918.2
• UniProt Accession #: P51530
• Molecular Weight: Observed MW: 127kDa
• NCBI Official Full Name: DNA replication ATP-dependent helicase/nuclease DNA2
• NCBI Official Synonym Full Names: DNA replication helicase/nuclease 2
• NCBI Official Symbol: DNA2
• NCBI Official Synonym Symbols: DNA2L; hDNA2
• NCBI Protein Information: DNA replication ATP-dependent helicase/nuclease DNA2
• UniProt Protein Name: DNA replication ATP-dependent helicase/nuclease DNA2
• Protein Family: DNA replication ATP-dependent helicase/nuclease
• UniProt Gene Name: DNA2
• UniProt Synonym Gene Names: DNA2L; KIAA0083; hDNA2
• UniProt Entry Name: DNA2_HUMAN

NCBI Description

This gene encodes a member of the DNA2/NAM7 helicase family. The encoded protein is a conserved helicase/nuclease involved in the maintenance of mitochondrial and nuclear DNA stability. Mutations in this gene are associated with autosomal dominant progressive external ophthalmoplegia-6 (PEOA6) and Seckel syndrome 8. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2014]