Rabbit anti-Human DNA-PK Polyclonal Antibody | anti-DNA-PK antibody

DNA-PK (Phospho-Y2609) Polyclonal Antibody

Cat. Num. AAA3005537

• Gene Names: PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNAPKc; DNA-PKC; DNA-PKcs
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: DNA-PK; Polyclonal Antibody; DNA-PK (Phospho-Y2609) Polyclonal Antibody; DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNA-PKcs; DNPK1; p460; PRKDC; HYRC; HYRC1; anti-DNA-PK antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: p-DNA-PK (Y2609) polyclonal antibody detects endogenous levels of DNA-PK protein only when phosphorylated at Tyr2609.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 4097

• Applicable Applications for anti-DNA-PK antibody: Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200
• Immunogen: Synthetic phosphopeptide derived from human DNA-PK around the phosphorylation site of Tyrosine 2609.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of p-DNA-PK (Y2609) pAb in paraffin-embedded human liver carcinoma tissue at 1:100.)

Related Product Information for anti-DNA-PK antibody

The phosphatidylinositol kinase (PIK) family members fall into two distinct subgroups. The first subgroup contains proteins such as the PI 3- and PI 4-kinases and the second group comprises the PIK-related kinases. The PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in common a region of homology at their carboxy termini that is not present in the PI 3- and PI 4-kinases. All of the members of the PIK-related kinases are also over 270 kDa. The Atm gene is mutated in the autosomal recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar degeneration (ataxia) and the appearance of dilated blood vessels (telangiectases) in the conjunctivae of the eyes.

NCBI and Uniprot Product Information

• NCBI GI #: 126032350
• NCBI GeneID: 5591
• NCBI Accession #: NP_001075109.1
• NCBI GenBank Nucleotide #: NP_001075109.1
• UniProt Accession #: P78527
• Molecular Weight: ~ 450kDa
• NCBI Official Full Name: DNA-dependent protein kinase catalytic subunit isoform 2
• NCBI Official Synonym Full Names: protein kinase, DNA-activated, catalytic subunit
• NCBI Official Symbol: PRKDC
• NCBI Official Synonym Symbols: HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNAPKc; DNA-PKC; DNA-PKcs
• NCBI Protein Information: DNA-dependent protein kinase catalytic subunit
• UniProt Protein Name: DNA-dependent protein kinase catalytic subunit
• UniProt Gene Name: PRKDC
• UniProt Synonym Gene Names: HYRC; HYRC1; DNA-PK catalytic subunit; DNA-PKcs
• UniProt Entry Name: PRKDC_HUMAN

NCBI Description

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Uniprot Description

DNAPK: an atypical protein kinase of the PIKK family. Involved in DNA nonhomologous end joining (NHEJ) required for double-strand break (DSB) repair and V(D)J recombination. and modulation of transcription. Must be bound to DNA to express its catalytic properties. Promotes processing of hairpin DNA structures in V(D)J recombination by activation of the hairpin endonuclease artemis (DCLRE1C). The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, suggesting a further role in the maintenance of telomeric stability and the prevention of chromosomal end fusion. Also involved in modulation of transcription. Defects cause severe combined immune deficiency (SCID) which is characterized by a lack of mature functional lymphocytes and a high susceptibility to lethal opportunistic infections. Required for repair of radiation-induced dsDNA breaks. Loss of function in mice or horses leads to the SCID (severe combined immune deficiency) phenotype due to failure of immunoglobulin rearrangement. Target of mutation in mismatch repair-deficient colorectal cancer. Inhibitor: KU-7059. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Kinase, protein; Nucleolus; Protein kinase, atypical; EC 2.7.11.1; DNA repair, damage; Protein kinase, Ser/Thr (non-receptor); ATYPICAL group; PIKK family; DNAPK subfamily

Chromosomal Location of Human Ortholog: 8q11

Cellular Component: nucleoplasm; transcription factor complex; membrane; nucleolus; DNA-dependent protein kinase complex; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; enzyme binding; DNA binding; DNA-dependent protein kinase activity; transcription factor binding; ATP binding; protein kinase activity

Biological Process: positive regulation of apoptosis; heart development; germ cell programmed cell death; T cell differentiation in the thymus; rhythmic process; double-strand break repair via nonhomologous end joining; negative regulation of protein amino acid phosphorylation; double-strand break repair; positive regulation of interferon type I production; response to gamma radiation; telomere maintenance; pro-B cell differentiation; somitogenesis; protein destabilization; immunoglobulin V(D)J recombination; B cell lineage commitment; protein modification process; regulation of circadian rhythm; DNA repair; double-strand break repair via homologous recombination; T cell lineage commitment; peptidyl-serine phosphorylation; DNA damage response, signal transduction resulting in induction of apoptosis; cellular response to insulin stimulus; innate immune response; T cell receptor V(D)J recombination; positive regulation of transcription from RNA polymerase II promoter; brain development

Disease: Immunodeficiency 26 With Or Without Neurologic Abnormalities