Rabbit anti-Human, Mouse DNA Methyltransferase 3B Polyclonal Antibody | anti-DNMT3B antibody

DNA Methyltransferase 3B (Dnmt 3B)

Cat. Num. AAA610504

• Gene Names: DNMT3B; ICF; M.HsaIIIB
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunoprecipitation
• Purity: Serum; Serum
• Synonyms: DNA Methyltransferase 3B; Polyclonal Antibody; DNA Methyltransferase 3B (Dnmt 3B); Anti -DNA Methyltransferase 3B (Dnmt 3B); anti-DNMT3B antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: This antibody is specific for DNMT3b. Species Crossreactivity: Reacts with human and mouse protein. Other species have not been tested.
• Purity/Purification: Serum; Serum

• Applicable Applications for anti-DNMT3B antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.
• Immunogen: This antibody was raised against residues 4-101 of the human DNMT3b protein.
• Positive Control: HCT116 cell lysates.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-DNMT3B antibody

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

Product Categories/Family for anti-DNMT3B antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 6118092
• NCBI GeneID: 1789
• UniProt Accession #: Q2PJS8; Q59H79; Q9UBC3
• Molecular Weight: 39,451 Da
• NCBI Official Full Name: DNA cytosine-5 methyltransferase 3B
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 3 beta
• NCBI Official Symbol: DNMT3B
• NCBI Official Synonym Symbols: ICF; M.HsaIIIB
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3B; DNA (cytosine-5)-methyltransferase 3B; DNA MTase HsaIIIB; OTTHUMP00000030604; OTTHUMP00000030605; OTTHUMP00000030606; OTTHUMP00000164397; DNA methyltransferase HsaIIIB
• UniProt Protein Name: DNA methyltransferase
• Protein Family: DNA
• UniProt Gene Name: DNMT3B
• UniProt Entry Name: Q2PJS8_HUMAN

NCBI Description

CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF) syndrome. Eight alternatively spliced transcript variants have been described. The full length sequences of variants 4 and 5 have not been determined. [provided by RefSeq]

Uniprot Description

DNMT3B iso3: a ubiquitous DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Numerous cancer cell lines and primary acute leukemias express aberrant DNMT3B transcripts, especially DNMT3B7. Transfection of DNMT3B7 into 293 cells alters the pattern of genes expressed in the transfected cells. Can interact with DNMT1, which might be a co-operative event during DNA methylation. Methylates CpG sites at a rate slower than DNMT3A and much slower than DNMT1. Interacts with SUV39H1, SETDB1, SUMO1 and UBE2I9. Interacts with DNMT1 and DNMT3A. Mutations in DNMT3B have been shown to cause immunodeficiency-centromeric instability-facial anomalies syndrome (ICF). Six alternatively spliced isoforms of the human protein have been reported. Isoform 1 is expressed in all tissues except brain, skeletal muscle and PBMC, 3 is ubiquitous, 4 is expressed in all tissues except brain, skeletal muscle, lung and prostate and 5 is detectable only in testis and at very low level in brain and prostate. Isoforms 4 and 5 are probably not enzymatically active due to the deletion of two conserved methyltransferase motifs.

Protein type: Methyltransferase, DNA; EC 2.1.1.37; Cell development/differentiation; Methyltransferase; Amino Acid Metabolism - cysteine and methionine

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear heterochromatin; nucleus

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; protein binding; unmethylated CpG binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; metal ion binding; transcription corepressor activity; DNA-methyltransferase activity

Biological Process: negative regulation of histone H3-K9 methylation; response to drug; genetic imprinting; S-adenosylhomocysteine metabolic process; positive regulation of histone H3-K4 methylation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; cytosine methylation within a CG sequence; protein complex localization; methylation-dependent chromatin silencing; negative regulation of gene expression, epigenetic; DNA methylation; S-adenosylmethioninamine metabolic process; gene expression; response to ionizing radiation; positive regulation of neuron differentiation; DNA methylation on cytosine

Disease: Immunodeficiency-centromeric Instability-facial Anomalies Syndrome 1