Rabbit anti-Human DMP1 Polyclonal Antibody | anti-DMP1 antibody

DMP1, NT (DMP1, Dentin matrix acidic phosphoprotein 1) (APC)

Cat. Num. AAA6292433

• Gene Names: DMP1; ARHP; ARHR; DMP-1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DMP1; Polyclonal Antibody; NT (DMP1; Dentin matrix acidic phosphoprotein 1) (APC); Dentin matrix acidic phosphoprotein 1; anti-DMP1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-DMP1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DMP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 130-159 amino acids from the N-terminal region of human DMP1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DMP1 Antibody (N-term) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the DMP1 antibody detected the DMP1 protein (arrow).)

Related Product Information for anti-DMP1 antibody

Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene.

Product Categories/Family for anti-DMP1 antibody

Antibodies; Proteins

References

Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010). Turan, S., et al. Bone 46(2):402-409(2010). Pereira, R.C., et al. Bone 45(6):1161-1168(2009). Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 121256621
• NCBI GeneID: 1758
• NCBI Accession #: NP_001073380.1
• NCBI GenBank Nucleotide #: NM_001079911.2
• UniProt Accession #: Q13316; O43265; A1L4L3
• Molecular Weight: 55,782 Da
• NCBI Official Full Name: dentin matrix acidic phosphoprotein 1 isoform 2
• NCBI Official Synonym Full Names: dentin matrix acidic phosphoprotein 1
• NCBI Official Symbol: DMP1
• NCBI Official Synonym Symbols: ARHP; ARHR; DMP-1
• NCBI Protein Information: dentin matrix acidic phosphoprotein 1; dentin matrix protein 1
• UniProt Protein Name: Dentin matrix acidic phosphoprotein 1
• Protein Family: Dentin matrix acidic phosphoprotein
• UniProt Gene Name: DMP1
• UniProt Synonym Gene Names: DMP-1; Dentin matrix protein 1
• UniProt Entry Name: DMP1_HUMAN

NCBI Description

Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May have a dual function during osteoblast differentiation. In the nucleus of undifferentiated osteoblasts, unphosphorylated form acts as a transcriptional component for activation of osteoblast-specific genes like osteocalcin. During the osteoblast to osteocyte transition phase it is phosphorylated and exported into the extracellular matrix, where it regulates nucleation of hydroxyapatite.1 PublicationManual assertion based on experiment in:Ref.6