Rabbit anti-Human DLL3 Polyclonal Antibody | anti-DLL3 antibody

DLL3, CT (DLL3, Delta-like protein 3, Drosophila Delta homolog 3) (AP)

Cat. Num. AAA6292355

• Gene Names: DLL3; SCDO1
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: DLL3; Polyclonal Antibody; CT (DLL3; Delta-like protein 3; Drosophila Delta homolog 3) (AP); Drosophila Delta homolog 3; anti-DLL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DLL3.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-DLL3 antibody: Immunohistochemistry (IHC) Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 545-573 in the C-terminal region of human DLL3, UniProt Accession #Q9NYJ7.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis in 293 cell line lysates (35ug/lane) usingMBS6002036. This demonstrates MBS6002036 detected the DLL3 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6002036 followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MBS6002036 for immunohistochemistry. Clinical relevance has not been evaluated.)

Testing Data

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Product Categories/Family for anti-DLL3 antibody

Antibodies; Notch Proteins

References

Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009). Heuss, S.F., et al. Proc. Natl. Acad. Sci. U.S.A. 105(32):11212-11217(2008). Maisenbacher, M.K., et al. Hum. Genet. 116(5):416-419(2005). Whittock, N.V., et al. Clin. Genet. 66(1):67-72(2004). Bonafe, L., et al. Clin. Genet. 64(1):28-35(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 8393264
• NCBI GeneID: 10683
• NCBI Accession #: NP_058637.1
• NCBI GenBank Nucleotide #: NM_016941.3
• UniProt Accession #: Q9NYJ7; Q8NBS4; E9PFG2
• Molecular Weight: 61,178 Da
• NCBI Official Full Name: delta-like protein 3 isoform 1
• NCBI Official Synonym Full Names: delta-like 3 (Drosophila)
• NCBI Official Symbol: DLL3
• NCBI Official Synonym Symbols: SCDO1
• NCBI Protein Information: delta-like protein 3; delta3; drosophila Delta homolog 3
• UniProt Protein Name: Delta-like protein 3
• Protein Family: Delta-like protein
• UniProt Gene Name: DLL3
• UniProt Synonym Gene Names: Delta3

NCBI Description

This gene encodes a member of the delta protein ligand family. This family functions as Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain. Mutations in this gene cause autosomal recessive spondylocostal dysostosis 1. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm ().