Rabbit anti-Human Dkk-2 Polyclonal Antibody | anti-Dkk-2 antibody

Dkk-2, NT (Dickkopf Related Protein-2, Dickkopf-2, DKK-2, UNQ682/PRO1316) (FITC)

Cat. Num. AAA6472584

• Gene Names: DKK2; DKK-2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Dkk-2; Polyclonal Antibody; NT (Dickkopf Related Protein-2; Dickkopf-2; DKK-2; UNQ682/PRO1316) (FITC); anti-Dkk-2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DKK2.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-Dkk-2 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human DKK2 (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-DKK2 Pab is used in Western blot to detect DKK2 in Jurkat cell lysate.)

Western Blot (WB)

(Western blot analysis of DKK2 (arrow) using DKK2 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the DKK2 gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Western Blot (WB)

(DKK2 Antibody (T46) western blot analysis in mouse brain tissue lysates (35ug/lane).This demonstrates the DKK2 antibody detected the DKK2 protein (arrow).)

Product Categories/Family for anti-Dkk-2 antibody

Antibodies; Dickkopf Proteins

References

1. Clark, H.F., et al., Genome Res. 13(10):2265-2270 (2003). 2. Brott, B.K., et al., Mol. Cell. Biol. 22(17):6100-6110 (2002). 3. Krupnik, V.E., et al., Gene 238(2):301-313 (1999).

NCBI and Uniprot Product Information

• NCBI GI #: 7657023
• NCBI GeneID: 27123
• NCBI Accession #: NP_055236.1
• NCBI GenBank Nucleotide #: NM_014421.2
• UniProt Accession #: Q9UBU2; Q9UIU3; A0AVE9; B2R6S7
• Molecular Weight: 28,447 Da
• NCBI Official Full Name: dickkopf-related protein 2
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 2
• NCBI Official Symbol: DKK2
• NCBI Official Synonym Symbols: DKK-2
• NCBI Protein Information: dickkopf-related protein 2; hDkk-2; dickkopf-2; dickkopf 2 homolog; dickkopf homolog 2; dickkopf related protein-2
• UniProt Protein Name: Dickkopf-related protein 2
• UniProt Gene Name: DKK2
• UniProt Synonym Gene Names: Dickkopf-2; Dkk-2; hDkk-2
• UniProt Entry Name: DKK2_HUMAN

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. Activity of this protein is also modulated by binding to the Wnt co-receptor LDL-receptor related protein 6 (LRP6). [provided by RefSeq, Jul 2008]

Uniprot Description

DKK2: Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero- posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. Interacts with LRP5 and LRP6. Expressed in heart, brain, skeletal muscle and lung. Belongs to the dickkopf family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q25

Cellular Component: extracellular space

Biological Process: Wnt receptor signaling pathway; multicellular organismal development