Western Blot (WB)
(Western blot analysis of DJ-1 expression in HeLa (A), Jurkat (B), COLO205 (C), PC12 (D) whole cell lysates.)
Rabbit DJ-1 Polyclonal Antibody | anti-PARK7 antibody
Anti-DJ-1 Antibody
Western Blot (WB)
(Western blot analysis of DJ-1 expression in HeLa (A), Jurkat (B), COLO205 (C), PC12 (D) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of DJ-1 expression in HeLa (A), Jurkat (B), COLO205 (C), PC12 (D) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of DJ-1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of DJ-1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of DJ-1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of DJ-1 staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the peptidase C56 family of proteins. It acts as a positive regulator of androgen receptor-dependent transcription. It may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death. Defects in this gene are the cause of autosomal recessive early-onset Parkinson disease 7. Two transcript variants encoding the same protein have been identified for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
DJ-1: associated with autosomal recessive early onset parkinsonism. Involved in the oxidative stress response. Three cysteines in DJ-1 may be oxidized to cysteine sulphonic acid in the cellular response to H2O2. Loss of DJ-1 function may lead to neurodegeneration.
Protein type: Transcription regulation; Tumor suppressor; EC 3.4.-.-; Nuclear receptor co-regulator
Chromosomal Location of Human Ortholog: 1p36.23
Cellular Component: PML body; mitochondrion; axon; mitochondrial matrix; cytoplasm; plasma membrane; mitochondrial intermembrane space; chromatin; nucleus; cytosol; mitochondrial respiratory chain complex I; lipid raft
Molecular Function: identical protein binding; oxidoreductase activity, acting on peroxide as acceptor; protein homodimerization activity; transcription coactivator activity; mercury ion binding; transcription factor binding; peptidase activity; mRNA binding; protein binding; copper ion binding; enzyme binding; peroxiredoxin activity; androgen receptor binding; cytokine binding; double-stranded DNA binding; glyoxalase III activity; superoxide dismutase copper chaperone activity; kinase binding; single-stranded DNA binding; receptor binding
Biological Process: negative regulation of protein export from nucleus; adult locomotory behavior; protein deglycosylation; membrane hyperpolarization; proteolysis; glycolate biosynthetic process; positive regulation of interleukin-8 production; negative regulation of protein amino acid phosphorylation; regulation of mitochondrial membrane potential; single fertilization; regulation of neuron apoptosis; enzyme active site formation via L-cysteine sulfinic acid; dopamine uptake; negative regulation of neuron apoptosis; negative regulation of protein binding; inflammatory response; mitochondrion organization and biogenesis; protein stabilization; activation of protein kinase B; regulation of TRAIL receptor biosynthetic process; detoxification of copper ion; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of peptidyl-serine phosphorylation; negative regulation of protein sumoylation; methylglyoxal catabolic process to D-lactate; membrane depolarization; positive regulation of protein kinase B signaling cascade; detoxification of mercury ion; regulation of inflammatory response; Ras protein signal transduction; negative regulation of ubiquitin-protein ligase activity; hydrogen peroxide metabolic process; negative regulation of protein kinase activity; lactate biosynthetic process; autophagy; negative regulation of protein ubiquitination; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; negative regulation of apoptosis
Disease: Parkinson Disease 7, Autosomal Recessive Early-onset
Research Articles on PARK7
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Product Notes
The PARK7 park7 (Catalog #AAA822224) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-DJ-1 Antibody reacts with Human, Rat, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's DJ-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/ICC (1/100 - 1/500), IP (1/10 - 1/100). Researchers should empirically determine the suitability of the PARK7 park7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DJ-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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