Rabbit anti-Human DISC1 Polyclonal Antibody | anti-DISC1 antibody

DISC1, CT (Disrupted In Schizophrenia 1, KIAA0457)

Cat. Num. AAA646778

• Gene Names: DISC1; SCZD9; C1orf136
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: DISC1; Polyclonal Antibody; CT (Disrupted In Schizophrenia 1; KIAA0457); Anti -DISC1; anti-DISC1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human DISC1.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.01% sodium azide, 50% glycerol.

• Applicable Applications for anti-DISC1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Western Blot: 1ug/ml; Immunohistochemistry (Formalin-fixed, paraffin-embedded): 10-20ug/ml
• Immunogen: Synthetic peptide corresponding to C-terminal residues of human DISC1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Brain, Cortex (formalin-fixed, paraffin-embedded) stained with DISC1 antibody at 10-20ug/ml followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-DISC1 antibody

Disrupted in Schizophrenia 1 (DISC1) is a gene whose mutant truncation is associated with major psychiatric illness with a predominance of schizophrenic symptomatology. DISC1 has been shown to interact with multiple proteins of the centrosome and cytoskeletal system including MIPT3, MAP1A and NUDEL; proteins that localize receptor to membranes, including ACTN2 and beta-4-spectrin; and proteins that transducer signals from membrane receptors. This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for anti-DISC1 antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 257153482
• NCBI GeneID: 27185
• NCBI Accession #: NP_001158021.1
• NCBI GenBank Nucleotide #: NM_001164549.1
• UniProt Accession #: Q9NRI5; O75045; Q5VT44; A6NLH2; C4P091; C4P095; C4P0A1; C4P0A3; C4P0B3; C4P0B6; C4P0C1; C9J6D0
• Molecular Weight: 93,611 Da
• NCBI Official Full Name: disrupted in schizophrenia 1 protein isoform l
• NCBI Official Synonym Full Names: disrupted in schizophrenia 1
• NCBI Official Symbol: DISC1
• NCBI Official Synonym Symbols: SCZD9; C1orf136
• NCBI Protein Information: disrupted in schizophrenia 1 protein
• UniProt Protein Name: Disrupted in schizophrenia 1 protein
• Protein Family: Disrupted in schizophrenia
• UniProt Gene Name: DISC1
• UniProt Synonym Gene Names: KIAA0457
• UniProt Entry Name: DISC1_HUMAN

NCBI Description

This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins. This gene is disrupted in a t(1;11)(q42.1;q14.3) translocation which segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Plays a role, together with PCNT, in the microtubule network formation. Ref.17 Ref.18 Ref.19

Subunit structure: Interacts with NDEL1. Interacts with CCDC88A (via C-terminus); the interaction is direct. Interacts with GSK3B

By similarity. Interacts with tubulin alpha, ACTN2, ANKHD1, ATF4, ATF5, CEP63, EIF3S3, MAP1A, NDEL1, PAFAH1B1, RANBP9, SPTBN4, SYNE1 and TRAF3IP1. Interaction with microtubules may be mediated in part by TRAF3IP1. Interacts (via C-terminal) with PCNT. Interacts with CHCHD6. Ref.7 Ref.9 Ref.12 Ref.16 Ref.17 Ref.20

Subcellular location: Cytoplasm. Cytoplasm › cytoskeleton. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cell junction › synapse › postsynaptic cell membrane › postsynaptic density

By similarity. Note: Colocalizes with NDEL1 in the perinuclear region and the centrosome

By similarity. Localizes to punctate cytoplasmic foci which overlap in part with mitochondria. Colocalizes with PCNT at the centrosome. Ref.7 Ref.9 Ref.12 Ref.13 Ref.17

Tissue specificity: Ubiquitous. Highly expressed in the dentate gyrus of the hippocampus. Also expressed in the temporal and parahippocampal cortices and cells of the white matter. Ref.15

Developmental stage: Expression rises within the dentate gyrus and temporal cortex from the neonatal period to infancy, declines markedly in adolescence, and declines further with aging. Ref.15

Involvement in disease: A chromosomal aberration involving DISC1 segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Translocation t(1;11)(q42.1;q14.3). The truncated DISC1 protein produced by this translocation is unable to interact with ATF4, ATF5 and NDEL1.Schizophrenia 9 (SCZD9) [MIM:604906]: A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.6 Ref.8 Ref.14

Sequence caution: The sequence BAA32302.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.The sequence CAH70955.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI15677.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI17204.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI21886.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI22543.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAI23013.1 differs from that shown. Reason: Erroneous gene model prediction.