Rabbit anti-Human DIRAS3 Polyclonal Antibody | anti-DIRAS3 antibody

DIRAS3 (DIRAS3, ARHI, NOEY2, RHOI, GTP-binding protein Di-Ras3, Distinct subgroup of the Ras family member 3, Rho-related GTP-binding protein RhoI) (FITC)

Cat. Num. AAA6292204

• Gene Names: DIRAS3; ARHI; NOEY2
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DIRAS3; Polyclonal Antibody; DIRAS3 (DIRAS3; ARHI; NOEY2; RHOI; GTP-binding protein Di-Ras3; Distinct subgroup of the Ras family member 3; Rho-related GTP-binding protein RhoI) (FITC); Rho-related GTP-binding protein RhoI; anti-DIRAS3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-DIRAS3 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:50-100; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DIRAS3 Antibody is generated from rabbits immunized with recombinant protein of human DIRAS3.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DIRAS3 Antibody western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the DIRAS3 antibody detected the DIRAS3 protein (arrow).)

Immunohistochemistry (IHC)

(DIRAS3 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DIRAS3 Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(DIRAS3 Antibody flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-DIRAS3 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 4757772
• NCBI GeneID: 9077
• NCBI Accession #: NP_004666.1
• NCBI GenBank Nucleotide #: NM_004675.2
• UniProt Accession #: O95661; B3KMP3
• Molecular Weight: 25,861 Da
• NCBI Official Full Name: GTP-binding protein Di-Ras3
• NCBI Official Synonym Full Names: DIRAS family, GTP-binding RAS-like 3
• NCBI Official Symbol: DIRAS3
• NCBI Official Synonym Symbols: ARHI; NOEY2
• NCBI Protein Information: GTP-binding protein Di-Ras3; ras homolog gene family, member I; rho-related GTP-binding protein RhoI; distinct subgroup of the Ras family member 3
• UniProt Protein Name: GTP-binding protein Di-Ras3
• Protein Family: GTP-binding protein
• UniProt Gene Name: DIRAS3
• UniProt Synonym Gene Names: ARHI; NOEY2; RHOI
• UniProt Entry Name: DIRA3_HUMAN

Uniprot Description

DIRAS3: a member of the ras superfamily, and is expressed in normal ovarian and breast epithelial cells, but not in ovarian and breast cancers. It is an imprinted gene, with monoallelic expression of the paternal allele, which is associated with growth suppression. Thus, this gene appears to be a putative tumor suppressor gene whose function is abrogated in ovarian and breast cancers. [provided by RefSeq, Oct 2010]

Protein type: G protein, monomeric, di-Ras; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: plasma membrane

Molecular Function: GTPase activity; protein binding; GTP binding

Biological Process: genetic imprinting; small GTPase mediated signal transduction; regulation of cyclin-dependent protein kinase activity