Goat Diphtheria toxin Polyclonal Antibody

Diphtheria toxin

Cat. Num. AAA324190

• Synonyms: Diphtheria toxin; Polyclonal Antibody; Diphtheria toxin Antiserum Products; anti-Diphtheria toxin antibody

• Host: Goat
• Clonality: Polyclonal
• Specificity: Purified diphtheria toxin/toxoid
• Form/Format: BIOTIN

• Application Notes: Titer: neat in gel techniques
• Immunogen: Purified diphtheria toxoid
• Reactivity Note: Demonstrated reactivity with purified toxin
• Antiserum Characterization: (neat)

Related Product Information for anti-Diphtheria toxin antibody

This product consists of purified IgG fraction of the above antiserum covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution. The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1% sodium azide preservative. No stabilizing proteins have been added. Possible applications for this product include avidin and streptavidinamplification systems for immunohistochemistry, ELISA, fluorescence microscopy and immunoblotting. In addition, this product may be used in place of neat antiserum in almost any appropriate antibody-based technique. Protein concentration is 4-5 mg/ml. Recommended short term (<6 months) storage is liquid at 2-8 degree C. For longer term storage, aliquot and freeze. Unit size is 1.0 ml.

Product Categories/Family for anti-Diphtheria toxin antibody

OMNITOPE Polyclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 118949
• NCBI Accession #: P00588
• NCBI GenBank Nucleotide #: P00588
• UniProt Accession #: P00588
• Molecular Weight: 61,602 Da
• NCBI Official Full Name: Diphtheria toxin
• UniProt Protein Name: Diphtheria toxin
• Protein Family: Diphtheria toxin
• UniProt Entry Name: DTX_CORBE

Uniprot Description

Function: Diphtheria toxin, produced by a phage infecting Corynebacterium diphtheriae, is a proenzyme that, after activation, catalyzes the covalent attachment of the ADP ribose moiety of NAD to eukaryotic elongation factor 2 (eEF-2). Fragment A is the catalytic portion responsible for enzymatic ADP-ribosylation of elongation factor 2, while fragment B is responsible for binding of toxin to cell receptors and entry of fragment A. Ref.5 Ref.6

Catalytic activity: NAD+ + peptide diphthamide = nicotinamide + peptide N-(ADP-D-ribosyl)diphthamide.

Enzyme regulation: Partially inhibited by 1,8-naphthalimide (NAP). Ref.6

Subunit structure: Homodimer.

Sequence caution: The sequence AAA32182.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.