Rabbit diaphanous homolog 1 Polyclonal Antibody | anti-EHI_118260 antibody
Rabbit anti-human diaphanous homolog 1 (Drosophila) polyclonal Antibody
Reactivity
Human,Mouse,Rat; Other species are not tested. Please decide the specificity by homology.
Applications
ELISA, Western Blot, Immunofluorescence
Purity
Antigen Affinity Purified
Synonyms
diaphanous homolog 1; Polyclonal Antibody; Rabbit anti-human diaphanous homolog 1 (Drosophila) polyclonal Antibody; diaphanous homolog 1 (Drosophila); DIAPH1; DFNA1; DIA1; DRF1; FLJ25265; LFHL1; hDIA1; anti-EHI_118260 antibody
Host
Rabbit
Reactivity
Human,Mouse,Rat; Other species are not tested. Please decide the specificity by homology.
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
1176
Applicable Applications for anti-EHI_118260 antibody
ELISA (EIA), Western Blot (WB), Immunofluorescence (IF)
Application Notes
ELISA; Use at an assay dependent dilution.
WB: 1:1000-1:10000
IF: 1:10-1:100 (Recommender dilutions)
Positive WB detected in: HeLa cells, COS-7 cells, human heart tissue, human skeletal muscle tissue, NIH/3T3 cells
Positive IF detected in : HepG2 cells, Hela cells
WB: 1:1000-1:10000
IF: 1:10-1:100 (Recommender dilutions)
Positive WB detected in: HeLa cells, COS-7 cells, human heart tissue, human skeletal muscle tissue, NIH/3T3 cells
Positive IF detected in : HepG2 cells, Hela cells
Immunogen
Human DIAP1
Conjugate
Non-conjugated
Storage Buffer
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-10886 / sc-10885 / sc-10888 / sc-28775
Related Product Information for anti-EHI_118260 antibody
Rabbit polyclonal to DIAPH1
Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration By similarity. Has neurite outgrowth promoting activity By similarity. In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape
Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration By similarity. Has neurite outgrowth promoting activity By similarity. In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
130,858 Da
NCBI Official Full Name
diaphanous protein, homolog 1
NCBI Official Symbol
EHI_118260
NCBI Protein Information
diaphanous protein, homolog 1
UniProt Protein Name
Diaphanous protein, homolog 1, putative
UniProt Gene Name
EHI_118260
UniProt Entry Name
C4LZ08_ENTHI
Similar Products
Product Notes
The EHI_118260 ehi_118260 (Catalog #AAA714432) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human diaphanous homolog 1 (Drosophila) polyclonal Antibody reacts with Human,Mouse,Rat; Other species are not tested. Please decide the specificity by homology. and may cross-react with other species as described in the data sheet. AAA Biotech's diaphanous homolog 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunofluorescence (IF). ELISA; Use at an assay dependent dilution. WB: 1:1000-1:10000 IF: 1:10-1:100 (Recommender dilutions) Positive WB detected in: HeLa cells, COS-7 cells, human heart tissue, human skeletal muscle tissue, NIH/3T3 cells Positive IF detected in : HepG2 cells, Hela cells. Researchers should empirically determine the suitability of the EHI_118260 ehi_118260 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "diaphanous homolog 1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
