Rabbit anti-Human DIAPH3 Polyclonal Antibody | anti-DIAPH3 antibody

DIAPH3, CT (DIAPH3, DIAP3, Protein diaphanous homolog 3, Diaphanous-related formin-3, MDia2) (Azide free) (HRP)

Cat. Num. AAA6292161

• Gene Names: DIAPH3; AN; DIA2; DRF3; AUNA1; NSDAN; diap3; mDia2
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DIAPH3; Polyclonal Antibody; CT (DIAPH3; DIAP3; Protein diaphanous homolog 3; Diaphanous-related formin-3; MDia2) (Azide free) (HRP); MDia2; anti-DIAPH3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DIAPH3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DIAPH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1060-1090 amino acids from the C-terminal region of human DIAPH3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(DIAPH3 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the DIAPH3 antibody detected the DIAPH3 protein (arrow).)

Related Product Information for anti-DIAPH3 antibody

DIAPH3 binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics (By similarity).

Product Categories/Family for anti-DIAPH3 antibody

Antibodies; Transcription Factors

References

DeWard, A.D., et al. J. Biol. Chem. 284(30):20061-20069(2009). Block, J., et al. J Microsc 231(3):506-517(2008). Beli, P., et al. Nat. Cell Biol. 10(7):849-857(2008). Yang, C., et al. PLoS Biol. 5 (11), E317 (2007): Gupton, S.L., et al. J. Cell. Sci. 120 (PT 19), 3475-3487 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 110225351
• NCBI GeneID: 81624
• NCBI Accession #: NP_001035982.1; NP_112194.2
• NCBI GenBank Nucleotide #: NM_001042517.1
• UniProt Accession #: Q9NSV4; Q18P99; Q18PA0; Q18PA1; Q2KPB6; Q3ZK23; Q5JTP8; Q5T2S7; Q5XKF6; A2A3B8; A2A3B9; A2A3C0
• Molecular Weight: 127,856 Da
• NCBI Official Full Name: protein diaphanous homolog 3 isoform a
• NCBI Official Synonym Full Names: diaphanous related formin 3
• NCBI Official Symbol: DIAPH3
• NCBI Official Synonym Symbols: AN; DIA2; DRF3; AUNA1; NSDAN; diap3; mDia2
• NCBI Protein Information: protein diaphanous homolog 3
• UniProt Protein Name: Protein diaphanous homolog 3
• Protein Family: Protein diaphanous
• UniProt Gene Name: DIAPH3
• UniProt Synonym Gene Names: DIAP3; DRF3
• UniProt Entry Name: DIAP3_HUMAN

NCBI Description

This gene encodes a member of the diaphanous subfamily of the formin family. Members of this family are involved in actin remodeling and regulate cell movement and adhesion. Mutations in this gene are associated with autosomal dominant auditory neuropathy 1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Uniprot Description

Diaphanous-3: Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Defects in DIAPH3 are the cause of auditory neuropathy, autosomal dominant, type 1 (AUNA1). A form of sensorineural hearing loss with absent or severely abnormal auditory brainstem response, in the presence of normal cochlear outer hair cell function and normal otoacoustic emissions. Auditory neuropathies result from a lesion in the area including the inner hair cells, connections between the inner hair cells and the cochlear branch of the auditory nerve, the auditory nerve itself and auditory pathways of the brainstem. A disease- causing mutation in the conserved 5'-UTR leads to increased protein expression (PubMed:20624953). Belongs to the formin homology family. Diaphanous subfamily. 7 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 13q21.2

Cellular Component: cytosol; nucleus

Molecular Function: Rho GTPase binding; actin binding

Biological Process: spermatogenesis; actin cytoskeleton organization and biogenesis

Disease: Auditory Neuropathy, Autosomal Dominant, 1