Immunofluorescence (IF)
(Fluorescent image of A549 cells stained with DHX9 Antibody (N-term). MBS9210437 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Rabbit DHX9 Polyclonal Antibody | anti-DHX9 antibody
DHX9 Antibody (N-term)
IHC~~1:25
WB~~1:1000
Immunofluorescence (IF)
(Fluorescent image of A549 cells stained with DHX9 Antibody (N-term). MBS9210437 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunofluorescence (IF)
(Fluorescent image of A549 cells stained with DHX9 Antibody (N-term). MBS9210437 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. colon section using DHX9 Antibody (N-term). MBS9210437 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. colon section using DHX9 Antibody (N-term). MBS9210437 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. breast section using DHX9 Antibody (N-term). MBS9210437 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded H. breast section using DHX9 Antibody (N-term). MBS9210437 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)
Western Blot (WB)
(DHX9 Antibody (N-term) western blot analysis in K562 and 293 cell line lysates (35ug/lane).This demonstrates the DHX9 antibody detected the DHX9 protein (arrow).)
Western Blot (WB)
(DHX9 Antibody (N-term) western blot analysis in K562 and 293 cell line lysates (35ug/lane).This demonstrates the DHX9 antibody detected the DHX9 protein (arrow).)
Abdelhaleem M.M., et al. J. Immunol. 156:2026-2035(1996).
Zhang S., et al. J. Biol. Chem. 272:11487-11494(1997).
Goshima N., et al. Nat. Methods 5:1011-1017(2008).
Gregory S.G., et al. Nature 441:315-321(2006).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.[provided by RefSeq, Feb 2010]
Uniprot Description
DDX9: a helicase that unwinds double-stranded DNA and RNA in a 3' to 5' direction. Interacts with the RNA-induced silencing complex (RISC) in human cells and functions in RISC loading. Generates multiple secondary structures that influence RNA-binding proteins. Has a dsRNA binding motif at the N-terminus, RGG-box at the carboxyl terminus, and a bidirectional nuclear transport domain of 110 amino acids at the carboxyl terminus. May play a role in X-linked gene expression.
Protein type: EC 3.6.4.13; RNA splicing; Helicase; Nuclear receptor co-regulator; Spliceosome; RNA processing; Nucleolus; RNA-binding
Chromosomal Location of Human Ortholog: 1q25
Cellular Component: nucleoplasm; centrosome; membrane; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus; cytosol
Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; ATP-dependent RNA helicase activity; ATP binding; RNA helicase activity
Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; circadian rhythm; osteoblast differentiation; RNA splicing; positive regulation of interferon type I production; innate immune response; gene expression; DNA duplex unwinding
Research Articles on DHX9
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Product Notes
The DHX9 dhx9 (Catalog #AAA9210437) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-30. The DHX9 Antibody (N-term) reacts with Human (Predicted Reactivity: Bovine) and may cross-react with other species as described in the data sheet. AAA Biotech's DHX9 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). IF~~1:25 IHC~~1:25 WB~~1:1000. Researchers should empirically determine the suitability of the DHX9 dhx9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DHX9, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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