Rabbit DHX9 Polyclonal Antibody | anti-DHX9 antibody

DHX9 Antibody (N-term)

Cat. Num. AAA9210437

• Gene Names: DHX9; LKP; RHA; DDX9; NDH2; NDHII
• Reactivity: Human (Predicted Reactivity: Bovine)
• Applications: Immunofluorescence, ELISA, Immunohistochemistry, Western Blot
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: DHX9; Polyclonal Antibody; DHX9 Antibody (N-term); ATP-dependent RNA helicase A; RHA; DEAH box protein 9; Leukophysin; LKP; Nuclear DNA helicase II; NDH II; DDX9; NDH2; anti-DHX9 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This DHX9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human DHX9.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 1-30
• Sequence Length: 1270

• Applicable Applications for anti-DHX9 antibody: Immunofluorescence (IF), ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF~~1:25; IHC~~1:25; WB~~1:1000
• Fucntion: Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD- mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms. Positively regulates HIV-1 LTR-directed gene expression.
• Cellular Location: Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 2 weeks. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunofluorescence (IF)

(Fluorescent image of A549 cells stained with DHX9 Antibody (N-term). MBS9210437 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H. colon section using DHX9 Antibody (N-term). MBS9210437 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded H. breast section using DHX9 Antibody (N-term). MBS9210437 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

Western Blot (WB)

(DHX9 Antibody (N-term) western blot analysis in K562 and 293 cell line lysates (35ug/lane).This demonstrates the DHX9 antibody detected the DHX9 protein (arrow).)

Related Product Information for anti-DHX9 antibody

Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.

Product Categories/Family for anti-DHX9 antibody

Crown Antibodies

References

Lee C.-G., et al. J. Biol. Chem. 268:16822-16830(1993).
Abdelhaleem M.M., et al. J. Immunol. 156:2026-2035(1996).
Zhang S., et al. J. Biol. Chem. 272:11487-11494(1997).
Goshima N., et al. Nat. Methods 5:1011-1017(2008).
Gregory S.G., et al. Nature 441:315-321(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 100913206
• NCBI GeneID: 1660
• NCBI Accession #: NP_001348.2
• NCBI GenBank Nucleotide #: NM_001357.4
• UniProt Accession #: Q08211; Q05CI5; Q12803; Q32Q22; Q5VY62; Q6PD69; Q99556; B2RNV4
• Molecular Weight: 140958
• NCBI Official Full Name: ATP-dependent RNA helicase A
• NCBI Official Synonym Full Names: DEAH (Asp-Glu-Ala-His) box helicase 9
• NCBI Official Symbol: DHX9
• NCBI Official Synonym Symbols: LKP; RHA; DDX9; NDH2; NDHII
• NCBI Protein Information: ATP-dependent RNA helicase A
• UniProt Protein Name: ATP-dependent RNA helicase A
• Protein Family: ATP-dependent RNA helicase
• UniProt Gene Name: DHX9
• UniProt Synonym Gene Names: DDX9; LKP; NDH2; RHA; LKP; NDH II
• UniProt Entry Name: DHX9_HUMAN

NCBI Description

This gene encodes a member of the DEAH-containing family of RNA helicases. The encoded protein is an enzyme that catalyzes the ATP-dependent unwinding of double-stranded RNA and DNA-RNA complexes. This protein localizes to both the nucleus and the cytoplasm and functions as a transcriptional regulator. This protein may also be involved in the expression and nuclear export of retroviral RNAs. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 11 and 13.[provided by RefSeq, Feb 2010]

Uniprot Description

DDX9: a helicase that unwinds double-stranded DNA and RNA in a 3' to 5' direction. Interacts with the RNA-induced silencing complex (RISC) in human cells and functions in RISC loading. Generates multiple secondary structures that influence RNA-binding proteins. Has a dsRNA binding motif at the N-terminus, RGG-box at the carboxyl terminus, and a bidirectional nuclear transport domain of 110 amino acids at the carboxyl terminus. May play a role in X-linked gene expression.

Protein type: EC 3.6.4.13; RNA splicing; Helicase; Nuclear receptor co-regulator; Spliceosome; RNA processing; Nucleolus; RNA-binding

Chromosomal Location of Human Ortholog: 1q25

Cellular Component: nucleoplasm; centrosome; membrane; cytoplasm; nucleolus; ribonucleoprotein complex; nucleus; cytosol

Molecular Function: ATP-dependent DNA helicase activity; protein binding; DNA binding; ATP-dependent RNA helicase activity; ATP binding; RNA helicase activity

Biological Process: RNA processing; nuclear mRNA splicing, via spliceosome; circadian rhythm; osteoblast differentiation; RNA splicing; positive regulation of interferon type I production; innate immune response; gene expression; DNA duplex unwinding